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DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Citation:
DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Determination of trehalose in Candida albicans by HPLC-MS
School of Pharmacy,Second Military Medical University,Shanghai 200433,China
2.
School of Pharmacy,Second Military Medical University,Shanghai 200433,China
Received Date: 2010-01-25
Rev Recd Date:
2010-03-08
Abstract
Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.
Abstract: Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.
DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Citation:
DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
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