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Volume 28 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(3): 186-188

DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Citation: DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
shu

Determination of trehalose in Candida albicans by HPLC-MS

  • 1.

    School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 2.

    School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • Received Date: 2010-01-25
  • Rev Recd Date: 2010-03-08
  • Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.
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Determination of trehalose in Candida albicans by HPLC-MS

  • 1. School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 2. School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • Received Date: 2010-01-25
  • Rev Recd Date: 2010-03-08

Abstract: Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.

DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Citation: DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
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