Determianation of plasma concentration of sodium ferulate by HPLC and study on its pharmacokinetics in Beagle dogs

Key words: 
• Sodium ferulate /
• HPLC /
• Beagle dog /
• pharmacokinetics

Abstract: Objective :To establish a high-performance liquid chromatography method for analysis of sodium ferulate in Beagle dog plasma and to study the pharmacokinetics of sodium ferulate tablets. Methods :Sodium ferulate in plasma was extracted with methyl alcohol,separated on a Kromasil C₁₈ column(200mm×4.6mm,5μm) and the peak height ratio of sodium ferulate to the internal standard tinidazole was measured.Plasma concentration of sodium ferulate was determined at 320 nm using 0.1% acetic acid solution:acetonitrile(80:20) as the mobile phase and the flow rate was 1.0 mL/min. Results :Sodium ferulate was linear in the range of 0.04～25.0 μg/mL(r=0.999 6).The recovery of sodium ferulate was in the range of 94.0%～103.6%.The ABSolute recovery was more than 70%.The relative standard deviations of intra-day and inter-days were less than 10%.The minimum detectable concentration of sodium ferulate was 4.12 ng/mL.The plasma concentration-time curve of sodium ferulate oral tablet was found to be two-compartment model,and t_1/2(α) was(0.208±0.045) h,t_1/2(β) was(2.228±0.927) h. Conclusion :The method is sensitive,specific for the quantitative determination of sodium ferulate in the plasma of Beagle dog.It is suitable for pharmacokinetics study of sodium ferulate.