Determination of sinomenine in Caulis Sinomenii by nonaqueous capilary electrophoresis

Key words: 
• Caulis sinomenii /
• sinomenine /
• nonaqueous capilary electrophoresis /
• determination

Abstract: Objective :To establish a method for determination of sinomenine in Caulis Sinomenii. Methods :Caulis Sinomenii was extracted with 70% alcohol after pulverized to powder.An untreated fused-silica capillary(48.5 cm×50μm,40cm effective length) was used,the running buffer was 30 mmol/L sodium acid phosphate,the solvent was water:methanol=14,the voltage was 25 kV,the temperature was 25 ℃,the internal standard was berberine hydrochloride,the ultraviolet wavelength was set at 210 nm,running time was 20 minute. Results :Sinomenine,internal standard and impurities were separated by baseline in above conditions,sinomenine was liner in the range of 34.66～346.6 μg/mL,linear equation was Y=0.029X-0.03,r=0.9998,the correlation was 0.9998,the limit of determination was 3.466 μg/mL(S/N=3),the limit of quantitation was 17.33 μg/mL(S/N=10),the average recovery was 100.1%(RSD=3.1%,n=6),RSD of intra-day and inter-day were lower than 5%(n=3). Conclusion :The method can be utilized for the determination of sinomenine in Caulis Sinomenii,it was the advantages of convenience,fastness,accuracy,low sample and reagent consumption.