Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 27 Issue 6
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(6): 430-433

CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.
Citation: CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.
shu

Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC

  • 1.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China

  • 2.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2009-02-26
  • Objective :To establish a RP-HPLC method for separation and determination of active constituents in Fructus Cnidii from 10 different regions,and evaluate the quality of the Fructus Cnidii. Methods :DiamonsilTM C18(150mm×4.6 mm,5μm) was used with a mobile phase of ammonium acetate buffer(pH 4.0)/acetonitrile(50/50,,v/v) and a flow rate of 1.0 mL/min,at the detection wavelength of 320 nm. Results :The calibration curves were linear in the range of 4～100μg/mL for bergapten(r=0.9998),4～100 μg/mL for imperatorin(r=0.999 6),10～200 μg/mL for osthole(r=0.999 7),respectively.The average recoveries were 98.1%,92.5%,98.4%,with the RSD of 4.58%,5.73%,4.51%,respectively. Conclusion :The method is rapid,simple and accurate,and it can be suitable for the separation and determination of bergapten,imperatorin and osthole in Fructus Cnidii,quality control and geoherbalism research of Fructus Cnidii.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2131) PDF downloads(366) Cited by()

Related
Proportional views

Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC

  • 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China
  • 2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2009-02-26

Abstract: Objective :To establish a RP-HPLC method for separation and determination of active constituents in Fructus Cnidii from 10 different regions,and evaluate the quality of the Fructus Cnidii. Methods :DiamonsilTM C18(150mm×4.6 mm,5μm) was used with a mobile phase of ammonium acetate buffer(pH 4.0)/acetonitrile(50/50,,v/v) and a flow rate of 1.0 mL/min,at the detection wavelength of 320 nm. Results :The calibration curves were linear in the range of 4～100μg/mL for bergapten(r=0.9998),4～100 μg/mL for imperatorin(r=0.999 6),10～200 μg/mL for osthole(r=0.999 7),respectively.The average recoveries were 98.1%,92.5%,98.4%,with the RSD of 4.58%,5.73%,4.51%,respectively. Conclusion :The method is rapid,simple and accurate,and it can be suitable for the separation and determination of bergapten,imperatorin and osthole in Fructus Cnidii,quality control and geoherbalism research of Fructus Cnidii.

CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.
Citation: CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return