Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 27 Issue 6
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(6): 421-425

YANG Juan, GUO Mei-Li. Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 421-425.
Citation: YANG Juan, GUO Mei-Li. Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 421-425.
shu

Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius

  • Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2009-06-18
  • Objective :To establishing an appropriate combination of restriction endonuclease and silver-staining system for cDNA-AFLP analysis in Carthamus tinctorius L,. Methods :The efficiency of combination of PstI/MseI and MseI/EcoRI is compared.Several key factors in silver-staining including the affiliation and dissection dosage in sequencing plate processing,TEMED dosage in gel,pre-electrophoresis,temperature factor,staining liquid configuration etc are researched and discussed. Results :Enzyme efficiency of PstI/MseI is higher than MseI/EcoRI.Sequencing plate should be cleaned thoroughly.TEMED volume should be moderate.30mins pre-electrophoresis should be carried out.Temperature should be constant through the whole electrophoresis process. Conclusion :This research has found a high effective restriction endonuclease combination can further discover the genetic background of safflower and has established an appropriate silver-staining system which has overcome several problems in staining process.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2183) PDF downloads(489) Cited by()

Related
Proportional views

Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius

  • Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2009-06-18

Abstract: Objective :To establishing an appropriate combination of restriction endonuclease and silver-staining system for cDNA-AFLP analysis in Carthamus tinctorius L,. Methods :The efficiency of combination of PstI/MseI and MseI/EcoRI is compared.Several key factors in silver-staining including the affiliation and dissection dosage in sequencing plate processing,TEMED dosage in gel,pre-electrophoresis,temperature factor,staining liquid configuration etc are researched and discussed. Results :Enzyme efficiency of PstI/MseI is higher than MseI/EcoRI.Sequencing plate should be cleaned thoroughly.TEMED volume should be moderate.30mins pre-electrophoresis should be carried out.Temperature should be constant through the whole electrophoresis process. Conclusion :This research has found a high effective restriction endonuclease combination can further discover the genetic background of safflower and has established an appropriate silver-staining system which has overcome several problems in staining process.

YANG Juan, GUO Mei-Li. Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 421-425.
Citation: YANG Juan, GUO Mei-Li. Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 421-425.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return