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Volume 27 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(3): 198-200

ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.
Citation: ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.
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Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC

  • 1.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Xinxing Medicine Co., Ltd., Shanghai 200135, China

  • 2.

    Shanghai Xinxing Medicine Co., Ltd., Shanghai 200135, China

  • 3.

    Department of Pharmacy, 113rd Hospital of PLA, Ningbo 315040, China

  • Received Date: 2008-12-31
  • Objective :To set up a method for determination of arginine and leucine in human fibrin sealant kit. Methods :A Shim-pack CLC-ODS column was used after pre-column derivatization with 2,4-dinitrobenzene fluoride,with the mobile phase of 50% acetonitrile and 0.05mol/L acetate buffer,and the UV detection wavelength was 362 nm.The content was analyzed by external standard method. Results :These two amino acid derivatives can be well separated under the chromatographic conditions.The average recovery was 102.8%(RSD=0.83%) for Arg,and 103.8%(RSD=0.92%) for Leu. Conclusion :The method is accurate,convenient,low-cost and suitable for determination of the two amino-acids in human fibrin sealant kit.
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Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC

  • 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Xinxing Medicine Co., Ltd., Shanghai 200135, China
  • 2. Shanghai Xinxing Medicine Co., Ltd., Shanghai 200135, China
  • 3. Department of Pharmacy, 113rd Hospital of PLA, Ningbo 315040, China
  • Received Date: 2008-12-31

Abstract: Objective :To set up a method for determination of arginine and leucine in human fibrin sealant kit. Methods :A Shim-pack CLC-ODS column was used after pre-column derivatization with 2,4-dinitrobenzene fluoride,with the mobile phase of 50% acetonitrile and 0.05mol/L acetate buffer,and the UV detection wavelength was 362 nm.The content was analyzed by external standard method. Results :These two amino acid derivatives can be well separated under the chromatographic conditions.The average recovery was 102.8%(RSD=0.83%) for Arg,and 103.8%(RSD=0.92%) for Leu. Conclusion :The method is accurate,convenient,low-cost and suitable for determination of the two amino-acids in human fibrin sealant kit.

ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.
Citation: ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.
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