Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS

Key words: 
• hawthorn leaves /
• HPLC-DAD/ESI-MS /
• chemical constituents

Abstract: Objective : To analyze the chemical constituents of hawthorn leaves by high-performance liquid chromatography(HPLC)-diode array detector/electrospray ionization-mass spectrometry(HPLC-DAD/ESI-MS). Methods : Dried hawthorn leaves were extracted under reflux with 60% ethanol and purified by D101 macroporous resin again.The chromatographic separation was performed on a YMC ODS-C₁₈ column(250 mm×4.6 mm i.d.;5 μm) with a mobile phase composed of aceonitrile-0.1% formic acid(1585 v/v),eluted at a flow rate of 0.6 mL/min,and UV detection was set at 254 nm.The negative ionization mode with needle voltage 3 500 V,capillary voltage-20V,gas(N₂)press 21 psi and temperature of the capillary 300 ℃ was selected.Relative molecular mass data acquisition was performed from m/z 300 to 800 in full MS scan mode. Results :Eleven of the major chemical constituents of hawthorn leaves were characterized based on their retention behavior obtained on-line by their UV spectra and the HPLC-DAD/ESI-MS. Conclusion :A rapid and efficient method for studying the chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS was established.