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LIU Chang-hai, ZHAO Liang, ZHANG Hai, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Analysis of optical impurity of ephedrine levorotatory by capillary electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 33-37.
Citation:
LIU Chang-hai, ZHAO Liang, ZHANG Hai, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Analysis of optical impurity of ephedrine levorotatory by capillary electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 33-37.
Analysis of optical impurity of ephedrine levorotatory by capillary electrophoresis
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China
Received Date: 2008-10-14
Abstract
Objective : To develop a method for purity test and enantioseparation of ephedrine levorotatory by capillary electrophoresis(CE). Methods : The cyclodextrin,concentration,pH,organic solvent were studied,and the results indicated that the enantiomer of ephedrin and ephedrine levorotatory were baseline separated under the following experimental conditions:uncoated fused silica capillary(75 μm × 68 cm),25 mmol/L Tris,2% HP-β-CD and 1% SBE-β-CD,pH was adjusted at 3.0 by phosphoric acid,and 15 kV separation voltage.The detection wavelength was set at 214 nm with the column temperature of 15 ℃. Results : Under the experimental conditions adopted,the baseline separation of ephedrin and ephedrine levorotatory was obtained. Conclusions : The proposed method is simple,reliable and can be used for routine purity test and chiral separation.
Abstract: Objective : To develop a method for purity test and enantioseparation of ephedrine levorotatory by capillary electrophoresis(CE). Methods : The cyclodextrin,concentration,pH,organic solvent were studied,and the results indicated that the enantiomer of ephedrin and ephedrine levorotatory were baseline separated under the following experimental conditions:uncoated fused silica capillary(75 μm × 68 cm),25 mmol/L Tris,2% HP-β-CD and 1% SBE-β-CD,pH was adjusted at 3.0 by phosphoric acid,and 15 kV separation voltage.The detection wavelength was set at 214 nm with the column temperature of 15 ℃. Results : Under the experimental conditions adopted,the baseline separation of ephedrin and ephedrine levorotatory was obtained. Conclusions : The proposed method is simple,reliable and can be used for routine purity test and chiral separation.
LIU Chang-hai, ZHAO Liang, ZHANG Hai, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Analysis of optical impurity of ephedrine levorotatory by capillary electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 33-37.
Citation:
LIU Chang-hai, ZHAO Liang, ZHANG Hai, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Analysis of optical impurity of ephedrine levorotatory by capillary electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 33-37.
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