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CHEN Zhu-kang, PENG Xin-sheng, CHEN Gui-liang. Study on determination of particulate matter in small volume injections and sterile powder for injection[J]. Journal of Pharmaceutical Practice and Service, 2004, (2): 88-91.
Citation:
JIANG Ren, LIU Gang, ZHANG Hua, CHANG Hua, TAN Sheng-jian. Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (6): 435-437.
Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC
Department of Pharmacy,Hospital 306 of PLA,Beijing 100101, China
Received Date: 2007-08-03
Abstract
Objective: To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid. Methods: HPLC system consisted an Agilent C18column（ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm）and a mixture of methanol-water-phosphoric acid（38:62:0.5）as mobile phase.The flow rate was 1.0 mL/min and was monitored at 278 nm. Results: The retention time of baicalin was 20.6 min.The regress equation for baicalin was Y=0.060 47X+1.131,r=0.999 9,and the linear range was 62.84～314.2μg/mL.The average recovery of baicalin was（n=6）103.3%,RSD 0.43%. Conclusion: The method was sensitive,time-saving and accurate.
Abstract: Objective: To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid. Methods: HPLC system consisted an Agilent C18column（ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm）and a mixture of methanol-water-phosphoric acid（38:62:0.5）as mobile phase.The flow rate was 1.0 mL/min and was monitored at 278 nm. Results: The retention time of baicalin was 20.6 min.The regress equation for baicalin was Y=0.060 47X+1.131,r=0.999 9,and the linear range was 62.84～314.2μg/mL.The average recovery of baicalin was（n=6）103.3%,RSD 0.43%. Conclusion: The method was sensitive,time-saving and accurate.
CHEN Zhu-kang, PENG Xin-sheng, CHEN Gui-liang. Study on determination of particulate matter in small volume injections and sterile powder for injection[J]. Journal of Pharmaceutical Practice and Service, 2004, (2): 88-91.
Citation:
JIANG Ren, LIU Gang, ZHANG Hua, CHANG Hua, TAN Sheng-jian. Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (6): 435-437.
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CHEN Zhu-kang, PENG Xin-sheng, CHEN Gui-liang. Study on determination of particulate matter in small volume injections and sterile powder for injection[J]. Journal of Pharmaceutical Practice and Service, 2004, (2): 88-91.
CHEN Zhu-kang, PENG Xin-sheng, CHEN Gui-liang. Study on determination of particulate matter in small volume injections and sterile powder for injection[J]. Journal of Pharmaceutical Practice and Service, 2004, (2): 88-91.
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