Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC

Key words: 
• HPLC /
• Xiaoer Runfei Oral liquid /
• baicalin

Abstract: Objective: To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid. Methods: HPLC system consisted an Agilent C₁₈column（ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm）and a mixture of methanol-water-phosphoric acid（38:62:0.5）as mobile phase.The flow rate was 1.0 mL/min and was monitored at 278 nm. Results: The retention time of baicalin was 20.6 min.The regress equation for baicalin was Y=0.060 47X+1.131,r=0.999 9,and the linear range was 62.84～314.2μg/mL.The average recovery of baicalin was（n=6）103.3%,RSD 0.43%. Conclusion: The method was sensitive,time-saving and accurate.