Determine of the Fengdima eye drops by RP-HPLCC

Key words: 
• RP-HPLC /
• delermination /
• tetracaine hydrochloride /
• ephedrine hydrochloride

Abstract: Objective: To establish a RP-HPLC method for determining the contents of tetracaine hydrochloride and ephedrine hydrochloride in Fengdima eye drops. Methods: A gradient elution RP-HPLC method was used with a C₁₈column（4.6 mm×250 mm, 5μm）,the mobile phase consisted of 0.05 mol/l,sodium dihydrogen phosphate-acetonitril （9:1,for A pump） and 0.05 mol/L sodi- um dihydrogen phosphate buffer-acetonitril （3:7,for B pump）;the determination wavelength was 218 nm. Results: The determination was not interfered by the exeipient.The linear range of tetracaine hydrochloride was 50.26～753.9μg/mL,r=1.000.The linear range of ephedrine hydrochloride was 51.38～770.7μg/mL,r=0.999 5.The average recovery were 100.25%,99.57% respectively, while RSD were 0.75%,0.91%.respectively. Conclusion:: The contents of tetracaine hydrochloride and ephedrine hydrochloride could be determined effectively at the same time by the proposed method,which is simple,accurate and can he applied for determining the contents of preparation interfered by honey.