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Volume 26 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2008  >  (4): 289-290,293

LIU Hai-ying, YANG Ning, HUANG Li, SHEN Zhen-hua, SU Fang. Determination of chloramphenicol alcoholic solution by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 289-290,293.
Citation: LIU Hai-ying, YANG Ning, HUANG Li, SHEN Zhen-hua, SU Fang. Determination of chloramphenicol alcoholic solution by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 289-290,293.
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Determination of chloramphenicol alcoholic solution by HPLC

  • Institute for Drug and Instrument Control of United Logistics Department of Beijing Command,PLA,Beijing 100071,China

  • Objective: To developed a method for determination of chloramphenicol alcoholic solution. Methods: HPLC method was carried out on a ZORBAX 80A Extend-C18 column（4.6 mm×150 mm,5μm） with UV detection at 278 nm.The mobile phase was acetonitrile-0.1% sodium heptanesulfonate（25:75） with a flow rate of 1.0 mL/min.The column temperture was 35℃and the inject volumes was 10μL.The content of chloramphenicol was calculated by external standardization method. Results: There was a good lin- ear relationship for chloramphenicol within the range of 24.74～247.4μg/mL（r=0.999 9）.The average recovery was 99.28% and RSD was 0.27% （n=9）. Conclusion:: TThe method is simple,specific and not interfered by excipients.It is accurate for quality control of chloramphenicol alcoholic solution.
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Determination of chloramphenicol alcoholic solution by HPLC

  • Institute for Drug and Instrument Control of United Logistics Department of Beijing Command,PLA,Beijing 100071,China

Abstract: Objective: To developed a method for determination of chloramphenicol alcoholic solution. Methods: HPLC method was carried out on a ZORBAX 80A Extend-C18 column（4.6 mm×150 mm,5μm） with UV detection at 278 nm.The mobile phase was acetonitrile-0.1% sodium heptanesulfonate（25:75） with a flow rate of 1.0 mL/min.The column temperture was 35℃and the inject volumes was 10μL.The content of chloramphenicol was calculated by external standardization method. Results: There was a good lin- ear relationship for chloramphenicol within the range of 24.74～247.4μg/mL（r=0.999 9）.The average recovery was 99.28% and RSD was 0.27% （n=9）. Conclusion:: TThe method is simple,specific and not interfered by excipients.It is accurate for quality control of chloramphenicol alcoholic solution.

LIU Hai-ying, YANG Ning, HUANG Li, SHEN Zhen-hua, SU Fang. Determination of chloramphenicol alcoholic solution by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 289-290,293.
Citation: LIU Hai-ying, YANG Ning, HUANG Li, SHEN Zhen-hua, SU Fang. Determination of chloramphenicol alcoholic solution by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 289-290,293.
shu

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