Determination of vitaminB₆,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC

Key words: 
• HPLC /
• compound benxiaonamin tablels /
• vitaminB6 /
• phenobarbital /
• chlorphenamine maleate /
• diazepa

Abstract: Objective: To develop an HPLC quantitative method for the determination of vitaminB6,phenobarbital,chlorphena- mine maleate and diazepam in compound benxiaonamin tablets. Methods: The chromatographic conditions include column C₁₈ （150 mm×4.6 mm,4.6μm）;mobile phase A:aeetonitrile-water-triethylamine（100:900:1,contain 0.01 mol/L Sodium Heptane, adjusted to pH 3.5 by acetic aeid）;mobile phase B:aeetonitrile,gradient elution:0～12 min,A:100%～70%,12～20 min,A: 70%.The flow rate was 1 mL/min and monitored at 260 nm. Results: The retention time of vitaminB₆,phenobarbilal,chlorphena- mine maleate and diazepam were 4.6 min,10.2 min,12.3 min and 14.3 min respectively.The regress equation for VitaminB₆ was y=251.4x+2.403,r=0.999 8,and the linear range was0.1684～2.021μg,phenobarbital y=145.8x+1.612,r=0.9999 and the linear range was 0.510 8～6.130μg;ehlorphenamine maleate y=697.3x-7.252,r=0.999 9,and the linear range was 0.122 8～1.474μg,diazepam y=2767x+17.75,r=0.999 8 and the linear range was 0.049 60～0.595 2μg.The average re- covery of vitaminB₆,phenobarbital,chlorphenamine maleate and diazepam were 100.1%,99.4%,99.3% and 100.7%;and RSD were 0.18%,0.13%,0.47% and 0.16% respectively. Conclusion:: The method was sensiteve,time-saving and accurate.