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Volume 25 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2007  >  (5): 334-336

LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
Citation: LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
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Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC

  • Department of Pharmacy, No.113 Hospital of PLA, Ningbo 315040, China

  • Received Date: 2006-08-27
  • Objective :To establish the determination method of the content of psoralen and isopsoralen in compound Buguzhi granule. Methods :A HPLC method was adopted.This analysis was carried on a column of Waters RP-C18 with a mobile phase of water-methanol at the ratio of 5050.The detection wavelength was 246nm. Results :Within the range of 1.88～30.10 μg/mL,psoralen assumed fine linear relation between sample size and response,the average recovery was 98.56%,and RSD was 1.83%;within the range of 1.52～24.30 μg/mL,isopsoralen assumed fine linear relation between sample size and response,the average recovery was 97.82%,and RSD was 2.01%. Conclusion :This Method is simple,reliable and accurate and can be used in the quality control of Compound Buguzhi granule.
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Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC

  • Department of Pharmacy, No.113 Hospital of PLA, Ningbo 315040, China
  • Received Date: 2006-08-27

Abstract: Objective :To establish the determination method of the content of psoralen and isopsoralen in compound Buguzhi granule. Methods :A HPLC method was adopted.This analysis was carried on a column of Waters RP-C18 with a mobile phase of water-methanol at the ratio of 5050.The detection wavelength was 246nm. Results :Within the range of 1.88～30.10 μg/mL,psoralen assumed fine linear relation between sample size and response,the average recovery was 98.56%,and RSD was 1.83%;within the range of 1.52～24.30 μg/mL,isopsoralen assumed fine linear relation between sample size and response,the average recovery was 97.82%,and RSD was 2.01%. Conclusion :This Method is simple,reliable and accurate and can be used in the quality control of Compound Buguzhi granule.

LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
Citation: LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
shu

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