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Volume 25 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2007  >  (5): 334-336

LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
Citation: LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
shu

Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC

  • Department of Pharmacy, No.113 Hospital of PLA, Ningbo 315040, China

  • Received Date: 2006-08-27
  • Objective :To establish the determination method of the content of psoralen and isopsoralen in compound Buguzhi granule. Methods :A HPLC method was adopted.This analysis was carried on a column of Waters RP-C18 with a mobile phase of water-methanol at the ratio of 5050.The detection wavelength was 246nm. Results :Within the range of 1.88～30.10 μg/mL,psoralen assumed fine linear relation between sample size and response,the average recovery was 98.56%,and RSD was 1.83%;within the range of 1.52～24.30 μg/mL,isopsoralen assumed fine linear relation between sample size and response,the average recovery was 97.82%,and RSD was 2.01%. Conclusion :This Method is simple,reliable and accurate and can be used in the quality control of Compound Buguzhi granule.
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 14.5 %其他: 14.5 %其他: 0.8 %其他: 0.8 %China: 0.5 %China: 0.5 %Germany: 0.2 %Germany: 0.2 %Korea Republic of: 0.2 %Korea Republic of: 0.2 %Malaysia: 0.2 %Malaysia: 0.2 %Saitama: 0.2 %Saitama: 0.2 %Singapore: 0.2 %Singapore: 0.2 %[]: 0.7 %[]: 0.7 %上海: 1.2 %上海: 1.2 %云浮: 0.2 %云浮: 0.2 %北京: 21.7 %北京: 21.7 %北海: 0.2 %北海: 0.2 %南充: 0.2 %南充: 0.2 %厦门: 0.2 %厦门: 0.2 %台州: 0.3 %台州: 0.3 %台湾省: 0.2 %台湾省: 0.2 %吉林: 0.2 %吉林: 0.2 %周口: 0.2 %周口: 0.2 %呼和浩特: 0.2 %呼和浩特: 0.2 %大庆: 0.5 %大庆: 0.5 %天津: 0.2 %天津: 0.2 %宝鸡: 0.2 %宝鸡: 0.2 %巴音郭楞: 0.2 %巴音郭楞: 0.2 %广州: 1.0 %广州: 1.0 %张家口: 0.2 %张家口: 0.2 %徐州: 0.2 %徐州: 0.2 %恩施: 0.2 %恩施: 0.2 %杭州: 0.5 %杭州: 0.5 %枣庄: 0.2 %枣庄: 0.2 %梧州: 0.2 %梧州: 0.2 %汕尾: 0.2 %汕尾: 0.2 %深圳: 0.2 %深圳: 0.2 %湖州: 0.2 %湖州: 0.2 %澳门: 0.2 %澳门: 0.2 %盐城: 0.3 %盐城: 0.3 %石家庄: 0.3 %石家庄: 0.3 %秦皇岛: 0.2 %秦皇岛: 0.2 %芒廷维尤: 12.2 %芒廷维尤: 12.2 %花卷: 0.2 %花卷: 0.2 %衢州: 0.3 %衢州: 0.3 %襄阳: 0.2 %襄阳: 0.2 %西宁: 38.7 %西宁: 38.7 %贵阳: 0.2 %贵阳: 0.2 %通化: 0.2 %通化: 0.2 %邯郸: 0.3 %邯郸: 0.3 %郑州: 0.2 %郑州: 0.2 %重庆: 0.5 %重庆: 0.5 %长春: 0.2 %长春: 0.2 %首尔: 0.2 %首尔: 0.2 %香港特别行政区: 0.2 %香港特别行政区: 0.2 %其他其他ChinaGermanyKorea Republic ofMalaysiaSaitamaSingapore[]上海云浮北京北海南充厦门台州台湾省吉林周口呼和浩特大庆天津宝鸡巴音郭楞广州张家口徐州恩施杭州枣庄梧州汕尾深圳湖州澳门盐城石家庄秦皇岛芒廷维尤花卷衢州襄阳西宁贵阳通化邯郸郑州重庆长春首尔香港特别行政区Highcharts.com
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Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC

  • Department of Pharmacy, No.113 Hospital of PLA, Ningbo 315040, China
  • Received Date: 2006-08-27

Abstract: Objective :To establish the determination method of the content of psoralen and isopsoralen in compound Buguzhi granule. Methods :A HPLC method was adopted.This analysis was carried on a column of Waters RP-C18 with a mobile phase of water-methanol at the ratio of 5050.The detection wavelength was 246nm. Results :Within the range of 1.88～30.10 μg/mL,psoralen assumed fine linear relation between sample size and response,the average recovery was 98.56%,and RSD was 1.83%;within the range of 1.52～24.30 μg/mL,isopsoralen assumed fine linear relation between sample size and response,the average recovery was 97.82%,and RSD was 2.01%. Conclusion :This Method is simple,reliable and accurate and can be used in the quality control of Compound Buguzhi granule.

LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
Citation: LIANG Ai-min, XU Li, CHEN Gang, TANG Fan. Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 334-336.
shu

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