Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC

Key words: 
• Salvianolic acid B /
• Danshsen leaves and stems /
• HPLC /
• pharmaceutical analysis

Abstract: Objective : To establish an HPLC method for determination of salvianolic acid B in Danshen leaves and stems,and compare the content of salvianolic acid B in Danshen leaves and stems of different species,different locations and growth stages,so as to supply some evidence for the further exploitation and utilization of Danshen leaves and stems reasonably. Methods : The determination was performed on Phenomenex C₁₈ column(250 mm×4.6 mm 5μm);The mobile phase consisted of acetonitrile-meon-0.25%orthophosphoric acid solution(10∶30∶60) with a flow-rate of 1.0 mL/min.The detecting wavelength was at 286 nm. Results : The calibration curves were good linear regression(r=0.999 9) with measurement ranges(0.488～8.54μg).The recoveries were 98.17%. Conclusions : The method was successfully applied to quantify salvianolic acid B in Danshsen leaves and stems,and salvianolic acid B cotents in Danshsen leaves and stems of different species were not at the same level;Salviae miltiorrhiza Bge contained the higher salvianolic acid B than Salvia miltiorrhiza bge.f.alba.The salvianolic acid B contents in Danshsen leaves and stems from Sishui was the highest in Augest among different locations and growth stages for the same species.