Determination of arginine in human fibrinogen by HPLC with precolumn derivatization
- Received Date: 2005-12-16
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Key words:
- arginine /
- alanine, HPLC /
- derivation /
- 2, 4-dinitrofluorobenzene /
Abstract: Objective: To develop a method for determination of arginine in human fibrinogen. Methods: The sample was mixed with alanine as the internal standard, derived by 2, 4-dinitrofluorobenzene.The mobil phase was composed of 50% acetonitrile-0.05 mol/L acetate buffer(35∶65).The UV detection wavelength was 362nm. Results: There was a good linear relationship with the range of 0.16～0.06mg/mL(r=0.9998), average recovery of arginine was 102.4%, RSD was 1.4%. Conclusion: This method is accu-rate, simple, cheap, and can be used for determination of arginine in human fibrinogen.
| Citation: | ZHAO Yan-hong, WANG Qiang, CHEN Zhao-mei. Determination of arginine in human fibrinogen by HPLC with precolumn derivatization[J]. Journal of Pharmaceutical Practice and Service, 2006, (5): 270-271.
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