Determination of the glycyrrhizic acid in compound anemone rivularis buccal tablets by HPLC

Key words: 
• compound anemone rivularis tablets /
• glycyrrhizic acid /
• HPLC

Abstract: Objective To establish a method to determine the Glycyrrhizic acid in Compound Anemone rivularis tablets. Methods Using HPLC with C₁₈ column and mobile phase of methanol-0.2mol/L ammonium acetate solution-glacial acetic acid(53:47:1) with flow rate of 1.0 mL/min, the detection wavelength was 250nm. Result The linear range was 0.0520. 468mg/mL for ammonium glycyrrhizinate (r=0.9996), good linearity, precision and recovery were obtained. Conclusion The method is simple, rapid and accurate and could be used for quality control of compound anemone rivularis tablets.