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XING Wang-xing, MI He-ming, CHENG Shi-jing, HE Xiang, WU Yu-tian. Analysis of the origin fungi of the traditional Chinese medicine, Hongqu from different producing areas with RAPD technique[J]. Journal of Pharmaceutical Practice and Service, 2001, (3): 163-165,153.
Citation:
XING Wang-xing, MI He-ming, CHENG Shi-jing, HE Xiang, WU Yu-tian. Analysis of the origin fungi of the traditional Chinese medicine, Hongqu from different producing areas with RAPD technique[J]. Journal of Pharmaceutical Practice and Service, 2001, (3): 163-165,153.
Analysis of the origin fungi of the traditional Chinese medicine, Hongqu from different producing areas with RAPD technique
Department of Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
The 117th Hospital of PLA, Hangzhou 310013, China
3.
Department of Science Research Second Military Medical University, Shanghai 200433, China
Received Date: 2001-02-27
Abstract
OBJECTIVE Establish a random amplified polymorphism DNA(RAPD) method for classification of origin fungi of the traditional Chinese medicine, Hongqu. METHODS The genome DNAs of the origin fungi of Hongqu from 14 different producing areas were extracted by CTAB. Amplification products were detected by agarose gel elect rophoresis and done clustering analysis using PHYLIP3.5c.RESULTS A characteristic pattern was produced dependent on one of the 10 screened random primers and the genome amplified.The diversities of the fingerprint patterns of Monascus exist obvious by which we can distinguish easily.CONCLUSION RAPD as-say technique is a useful tool to identify of Hongqu.
Abstract: OBJECTIVE Establish a random amplified polymorphism DNA(RAPD) method for classification of origin fungi of the traditional Chinese medicine, Hongqu. METHODS The genome DNAs of the origin fungi of Hongqu from 14 different producing areas were extracted by CTAB. Amplification products were detected by agarose gel elect rophoresis and done clustering analysis using PHYLIP3.5c.RESULTS A characteristic pattern was produced dependent on one of the 10 screened random primers and the genome amplified.The diversities of the fingerprint patterns of Monascus exist obvious by which we can distinguish easily.CONCLUSION RAPD as-say technique is a useful tool to identify of Hongqu.
XING Wang-xing, MI He-ming, CHENG Shi-jing, HE Xiang, WU Yu-tian. Analysis of the origin fungi of the traditional Chinese medicine, Hongqu from different producing areas with RAPD technique[J]. Journal of Pharmaceutical Practice and Service, 2001, (3): 163-165,153.
Citation:
XING Wang-xing, MI He-ming, CHENG Shi-jing, HE Xiang, WU Yu-tian. Analysis of the origin fungi of the traditional Chinese medicine, Hongqu from different producing areas with RAPD technique[J]. Journal of Pharmaceutical Practice and Service, 2001, (3): 163-165,153.
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