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Volume 19 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2001  >  (1): 27-28,30

LIU Yon-ping, YANG Zhen-hua, Cao Yan-jun. RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository[J]. Journal of Pharmaceutical Practice and Service, 2001, (1): 27-28,30.
Citation: LIU Yon-ping, YANG Zhen-hua, Cao Yan-jun. RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository[J]. Journal of Pharmaceutical Practice and Service, 2001, (1): 27-28,30.
shu

RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository

  • 1.

    Department of Pharmacy, No.463 Hospital of PLA, Shenyang 110042, China

  • 2.

    Institute of Materia Medica, Shanxi Medical University, Taiyuan 030001, China

  • Received Date: 2000-07-08
  • OBJECTIVE To study resersed phase high performance liquid chromatographic method for the determination of berberine hydrochloride (Ber) in You Sai Qing suppository.METHODS Ber was chromatographed on a shim pack CLG-ODS column with a mobile phase consisting of H3PO4 (0.01mol/L):MeOH(AR)(65:35).The flow rate was 1.0ml/min and the monitoring wavelength is 279nm. RESULTS The calhration curve was linear in the range of 3～100μg/ml.The recovery was 99.4%,RSD was 1.49%.CONCLUSION The methed is simple, accurate and relief and Can be used to determine the Ber.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository

  • 1. Department of Pharmacy, No.463 Hospital of PLA, Shenyang 110042, China
  • 2. Institute of Materia Medica, Shanxi Medical University, Taiyuan 030001, China
  • Received Date: 2000-07-08

Abstract: OBJECTIVE To study resersed phase high performance liquid chromatographic method for the determination of berberine hydrochloride (Ber) in You Sai Qing suppository.METHODS Ber was chromatographed on a shim pack CLG-ODS column with a mobile phase consisting of H3PO4 (0.01mol/L):MeOH(AR)(65:35).The flow rate was 1.0ml/min and the monitoring wavelength is 279nm. RESULTS The calhration curve was linear in the range of 3～100μg/ml.The recovery was 99.4%,RSD was 1.49%.CONCLUSION The methed is simple, accurate and relief and Can be used to determine the Ber.

LIU Yon-ping, YANG Zhen-hua, Cao Yan-jun. RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository[J]. Journal of Pharmaceutical Practice and Service, 2001, (1): 27-28,30.
Citation: LIU Yon-ping, YANG Zhen-hua, Cao Yan-jun. RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository[J]. Journal of Pharmaceutical Practice and Service, 2001, (1): 27-28,30.
shu

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