Effects on myocardial ischemia re-perfusion injury treated by injection of Radix Cynanchi Panticulati (RCP)on Neiguan(PC6)acupoint in rat

Key words: 
• radix cynanchi paniculati /
• ischemia re-perfusion injury /
• calcium ion /
• hemodynamics /
• rat /
• PC6 acupoint injection

Abstract: OBJECTIVE To Study the effects on myocardial ischemia re-perfusion injury treated by RCP on PC6 acupoint in rat.METHODS Wistar rats were randomized into groups of RCP acupoint group(A); i.m. RCP group(B);verapamil group(C) and NS control group(D).Myocardial ischemia re-perfusion injury models were set up.The hemodynamics parameters of the blood pressure(BP),left ventricular end systolic pressure(LVESP),left ventricular end diastolic pressure(LVEDP) and±dp/dt_max were recorded during the experiment.The intracelluar free calcium of the serum platelets and myocardial cells were measured with fluorescent probe,fura 2 established 2h after myocardial ischemia re-perfusion injury models.RESULTS In group A and group C,the concentration of calcium ion in myocardial cell were lower than that in group D(P<0.01).Group A was higher than group D in BP,LVESP and was lower inLVEDP,-dp/dt_max.Difference of the up-mentioned indexes between group A and group C appeared to be insignificant.CONCLUSION PC6 acupoint injection by RCP might increase myocardial contractility and prevent calcium overlodad in myocardial ischemia reperfusion injury rat.