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Volume 14 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 1996  >  (5): 295-297

Tan Shengjian, Qiu Xia, Li Guanghui, Zhao Bingcheng, Liang Yuqing. Determination of puerarin in Gegen qinlian pian by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1996, (5): 295-297.
Citation: Tan Shengjian, Qiu Xia, Li Guanghui, Zhao Bingcheng, Liang Yuqing. Determination of puerarin in Gegen qinlian pian by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1996, (5): 295-297.
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Determination of puerarin in Gegen qinlian pian by RP-HPLC

  • 1.

    Institute for Drug Control of Commission of Science Technology and Industry for National Defence Beijing 100101

  • 2.

  • 3.

  • A quantitative method was developed for the determination of puerarin in Gegen Qing lian Pian by reversed phase HPLC.Chromatographic conditions included column ODS-C(10),column temperature,35℃;detector UV,250nm;mobile phase,methanol-water(23:77),flow rate 1 ml/min;injecting volume,10μl;external standad.the number of theoretical plates calculated by puerarin peak was no less than 2000.The standard curve was linear in the concentration range of 5μg/ml-80μg/ml,and correlation coefficient was 0.9999.The average rccovery and the relative standsrd deviation were 97.6% and 1.8%,respectively.
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通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of puerarin in Gegen qinlian pian by RP-HPLC

  • 1. Institute for Drug Control of Commission of Science Technology and Industry for National Defence Beijing 100101
  • 2. 
  • 3. 

Abstract: A quantitative method was developed for the determination of puerarin in Gegen Qing lian Pian by reversed phase HPLC.Chromatographic conditions included column ODS-C(10),column temperature,35℃;detector UV,250nm;mobile phase,methanol-water(23:77),flow rate 1 ml/min;injecting volume,10μl;external standad.the number of theoretical plates calculated by puerarin peak was no less than 2000.The standard curve was linear in the concentration range of 5μg/ml-80μg/ml,and correlation coefficient was 0.9999.The average rccovery and the relative standsrd deviation were 97.6% and 1.8%,respectively.

Tan Shengjian, Qiu Xia, Li Guanghui, Zhao Bingcheng, Liang Yuqing. Determination of puerarin in Gegen qinlian pian by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1996, (5): 295-297.
Citation: Tan Shengjian, Qiu Xia, Li Guanghui, Zhao Bingcheng, Liang Yuqing. Determination of puerarin in Gegen qinlian pian by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1996, (5): 295-297.
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