Simultaneous determination of six alkaloids in alkaloids extract of Radix Aconiti Praeparata (Fuzi) by HPLC

LIU Min; ZHANG Hai; CAI Ya-mei; CHEN Xiao-fei; ZHU Zhen-yu; CHAI Yi-feng

doi:10.3969/j.issn.1006-0111.2013.03.005

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Objective To extract alkaloids in Radix Aconiti Praeparata (Fuzi) and determine the contents of six alkaloids benzoylmesaconine, benzoylaconine, benzoylhyaconine, mesaaconine, aconine and hyaconine by high performance liquid chromatography (HPLC). Methods The crude herb Fuzi was extracted by 85% ethanol, and separated by macroporous resin column. HPLC condition was as follows:Chromatographic column:Waters Terra C₁₈ (3.0 mm×100 mm, 3.5 μm); Mobile phase:A was acetonitrile, B was water (5 mM ammonium acetate), with gradient elution, the gradient of A phase:15%~26%(0~10 min), 26%~30%(10~20 min), 30%(20~25 min); flowing rate:0.5 ml/min; detection wave:23 5nm; column temperature:25℃; injection volume:5 μl. Results Six alkaloids, including benzoylmesaconine, benzoylaconine, benzoylhyaconine, mesaconine, aconine and hyaconine were separated at baseline within 25 min with good linear (r>0.999 0) between 11.40~114.0, 10.22~102.2, 10.36~103.6, 6.250~62.50, 13.50~135.0, 15.12~151.2 μg/ml. The result of intra-day and inter-day precisions, limits of detection and quantitation were at normal range. Each of the recovery rates (n=6) was 104.52% (RSD=2.0%), 103.43%(RSD=1.6%), 101.92%(RSD=2.3%), 99.45%(RSD=2.7%), 97.67%(RSD=1.9%), 101.15%(RSD=3.4%). The contents of six alkanoids in Fuzi alkaloids extract were determined. Conclusion The method was rapid, simple, reliable and could be used to determine six alkanoids in Fuzi alkanoids extract.

Simultaneous determination of six alkaloids in alkaloids extract of Radix Aconiti Praeparata (Fuzi) by HPLC

1. The Institute of Quality Control of Medical Material and Equipment under the Joint Logistic Department of Nanjing Command, Nanjing 210002, China

2. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

3. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2013-01-13

Rev Recd Date: 2013-04-26

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Abstract: Objective To extract alkaloids in Radix Aconiti Praeparata (Fuzi) and determine the contents of six alkaloids benzoylmesaconine, benzoylaconine, benzoylhyaconine, mesaaconine, aconine and hyaconine by high performance liquid chromatography (HPLC). Methods The crude herb Fuzi was extracted by 85% ethanol, and separated by macroporous resin column. HPLC condition was as follows:Chromatographic column:Waters Terra C₁₈ (3.0 mm×100 mm, 3.5 μm); Mobile phase:A was acetonitrile, B was water (5 mM ammonium acetate), with gradient elution, the gradient of A phase:15%~26%(0~10 min), 26%~30%(10~20 min), 30%(20~25 min); flowing rate:0.5 ml/min; detection wave:23 5nm; column temperature:25℃; injection volume:5 μl. Results Six alkaloids, including benzoylmesaconine, benzoylaconine, benzoylhyaconine, mesaconine, aconine and hyaconine were separated at baseline within 25 min with good linear (r>0.999 0) between 11.40~114.0, 10.22~102.2, 10.36~103.6, 6.250~62.50, 13.50~135.0, 15.12~151.2 μg/ml. The result of intra-day and inter-day precisions, limits of detection and quantitation were at normal range. Each of the recovery rates (n=6) was 104.52% (RSD=2.0%), 103.43%(RSD=1.6%), 101.92%(RSD=2.3%), 99.45%(RSD=2.7%), 97.67%(RSD=1.9%), 101.15%(RSD=3.4%). The contents of six alkanoids in Fuzi alkaloids extract were determined. Conclusion The method was rapid, simple, reliable and could be used to determine six alkanoids in Fuzi alkanoids extract.

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Simultaneous determination of six alkaloids in alkaloids extract of Radix Aconiti Praeparata (Fuzi) by HPLC

doi: 10.3969/j.issn.1006-0111.2013.03.005

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