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Volume 31 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(2): 113-115

HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
Citation: HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
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Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD

doi: 10.3969/j.issn.1006-0111.2013.02.008
  • 1.

    Changhai Hospital, Second Military Medical University, Department of Nursing

  • 2.

    Department of Pharmacy, Shanghai 200433, China

  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-07-24
  • Objective To establish a HPLC-ECD method for determination of dopamine hydrochloride in prefilled syringe. Methods The determination was performed on a Diamonsil-C18 analytical column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-0.11% sodium heptanesulfonate solution (containing 0.1 mmol/L EDTA-2Na and 5% glacial acetic acid, adjusting to pH 3.4 with 1 mol/L NaOH). The silver/silver chloride electrode was used as reference electrode and the glassy carbon electrode was used as the working electrode at the potential of+0. 65 V.The flow rate was 1.0 ml/min and column temperature was 20℃. Results The calibration curve of dopamine hydrochloride were linear in the concentration range of 0.05~5 μg/ml (r=0.999 9,n=5). The average recovery rate of dopamine hydrochloride was (99.19±4.03)% (n=3). RSD of intra-day and inter-day were less than 4% (n=3). The contents of dopamine hydrochlorid in three batches of samples were(102.97±1.11)%. Conclusion The method was accurate,rapid and high sensitivity,which was suitable for the determination of dopamine hydrochloride in prefilled syringe.
  • [1] 潘自红,贺国旭,胡小明,等. 分光光度法测定盐酸多巴胺的含量[J]. 分析试验室,2011,30(11):89.
    [2] Nishiyama J, Suzuki T, Murata T, et al. A questionnaire survey on operability of syringe pumps for prefilled syringes[J]. Tokai J Exp Clin Med, 2010, 35(3):95.
    [3] 中国药典2010版.二部[S].2010:688.
    [4] 吴燕琼,梁洁雯. HPLC测定盐酸多巴胺注射液的含量[J].广东药学,2005,15(3):15.
    [5] 宋才勇,余 莉,李雪艳,等. 微透析活体取样与高效液相色谱-电化学检测法联用测定帕金森大鼠脑中神经递质[J]. 分析试验室,2011,30(8):80.
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Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD

doi: 10.3969/j.issn.1006-0111.2013.02.008
  • 1. Changhai Hospital, Second Military Medical University, Department of Nursing
  • 2. Department of Pharmacy, Shanghai 200433, China
  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-07-24

Abstract: Objective To establish a HPLC-ECD method for determination of dopamine hydrochloride in prefilled syringe. Methods The determination was performed on a Diamonsil-C18 analytical column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-0.11% sodium heptanesulfonate solution (containing 0.1 mmol/L EDTA-2Na and 5% glacial acetic acid, adjusting to pH 3.4 with 1 mol/L NaOH). The silver/silver chloride electrode was used as reference electrode and the glassy carbon electrode was used as the working electrode at the potential of+0. 65 V.The flow rate was 1.0 ml/min and column temperature was 20℃. Results The calibration curve of dopamine hydrochloride were linear in the concentration range of 0.05~5 μg/ml (r=0.999 9,n=5). The average recovery rate of dopamine hydrochloride was (99.19±4.03)% (n=3). RSD of intra-day and inter-day were less than 4% (n=3). The contents of dopamine hydrochlorid in three batches of samples were(102.97±1.11)%. Conclusion The method was accurate,rapid and high sensitivity,which was suitable for the determination of dopamine hydrochloride in prefilled syringe.

HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
Citation: HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
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