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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(6): 451-453

WEI Shao-xuan, XIA Ai-jun, LIAO Hou-zhi. Determination of quercetin in Gnaphalium affine[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 451-453. doi: 10.3969/j.issn.1006-0111.2012.06.016
Citation: WEI Shao-xuan, XIA Ai-jun, LIAO Hou-zhi. Determination of quercetin in Gnaphalium affine[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 451-453. doi: 10.3969/j.issn.1006-0111.2012.06.016
shu

Determination of quercetin in Gnaphalium affine

doi: 10.3969/j.issn.1006-0111.2012.06.016
  • 1.

    Department of Traditional Chinese Medicine, School of Pharmacy, Guangxi Traditional Chinese Medical University, Nanning 530021, China

  • 2.

    Department of Pharmacy, the 303rd Hospital of PLA, Nanning 530021, China

  • 3.

    Guangxi Huike Institute of Materia Medica, Nanning 530007, China

  • Received Date: 2012-02-14
  • Rev Recd Date: 2012-04-24
  • Objective To establish the determination method of Quercetin content in Gnaphalium affine D. Don. Methods VP-ODS Cl8(4.6 mm×150 mm, 5 μm) colmnn was used, the mobile phase was methanol-0.2% phosphoric acid solution(45:55) with the flow rate as 1.0 ml/min. The detection wavelength was 375 nm. Results The linear relation of hyperin was good in the range from 10.22 μg/ml to 51.10 μg/ml. The average recovery was 100.18%, RSD was 1.38%. Conclusion The method established was accurate, sensitive and good in reproducibility which could be applied to the quality control of G. affine D. Don.
  • [1] 《全国中草药汇编》编写组.全国中草药汇编:上册[M].北京:人民卫生出版社,1996:894.
    [2] 国家中医药管理局,《中华本草》编委会.中华本草:第7册[M].上海:上海科学技术出版社,1999:848.
    [3] 李少锋,崔桂友.高效液相色谱法测定丁香中槲皮素的含量[J].扬州大学烹饪学报,2011, 28(1):51.
    [4] 王 丽,吕圭源,王 利.HPLC测定乌药叶中槲皮素的含量[J].北京联合大学学报:自然科学,2011,25(2):50.
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Determination of quercetin in Gnaphalium affine

doi: 10.3969/j.issn.1006-0111.2012.06.016
  • 1. Department of Traditional Chinese Medicine, School of Pharmacy, Guangxi Traditional Chinese Medical University, Nanning 530021, China
  • 2. Department of Pharmacy, the 303rd Hospital of PLA, Nanning 530021, China
  • 3. Guangxi Huike Institute of Materia Medica, Nanning 530007, China
  • Received Date: 2012-02-14
  • Rev Recd Date: 2012-04-24

Abstract: Objective To establish the determination method of Quercetin content in Gnaphalium affine D. Don. Methods VP-ODS Cl8(4.6 mm×150 mm, 5 μm) colmnn was used, the mobile phase was methanol-0.2% phosphoric acid solution(45:55) with the flow rate as 1.0 ml/min. The detection wavelength was 375 nm. Results The linear relation of hyperin was good in the range from 10.22 μg/ml to 51.10 μg/ml. The average recovery was 100.18%, RSD was 1.38%. Conclusion The method established was accurate, sensitive and good in reproducibility which could be applied to the quality control of G. affine D. Don.

WEI Shao-xuan, XIA Ai-jun, LIAO Hou-zhi. Determination of quercetin in Gnaphalium affine[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 451-453. doi: 10.3969/j.issn.1006-0111.2012.06.016
Citation: WEI Shao-xuan, XIA Ai-jun, LIAO Hou-zhi. Determination of quercetin in Gnaphalium affine[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 451-453. doi: 10.3969/j.issn.1006-0111.2012.06.016
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