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YIN Cha, WU Ting-ting, ZHU Dong-liang, LIU Zhi-rui, CHAI Yi-feng. Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010
Citation: YIN Cha, WU Ting-ting, ZHU Dong-liang, LIU Zhi-rui, CHAI Yi-feng. Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010

Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2012.06.010
  • Received Date: 2012-05-18
  • Rev Recd Date: 2012-06-26
  • Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of four major steroid glycosides (Timosaponin B, Timosaponin E1, Timosaponin BⅡ and Timosaponin AⅢ). Methods HPLC analysis was performed on an Agilent Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase of water (adjust to pH 3.3 by acetic acid) (A) and acetonitrile (B). The gradient elution program was as follow:0~8 min, 12%~23% B;8~25 min, 23% B;25~35 min, 23%~45% B;35~40 min, 45%~95% B. The temperature of drift tube was 55℃ and the nebulizer nitrogen flow rate was 4.0 Bar. Results The linearity was obtained over 42.10~252.6 μg/ml (r=0.999 3) for Timosaponin B, 48.80~292.8 μg/ml (r=0.999 1) for Timosaponin E1, 192.2~1153 μg/ml (r=0.999 7) for Timosaponin BⅡ and 8.512~85.12 μg/ml (r=0.998 5) for Timosaponin AⅢ. The RSDs of precision and stability of the samples were both less than 1% in 48 hours. The average recovery was between 96.04%~102.8%. Conclusions The present method, with satisfactory efficacy, was simple which could simultaneously determine multiple steroid glycosides in Anemarrhena asphodeloides Bge. from different areas.
  • [1] 中国药典2010版.一部[S]. 2010:196.
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    [3] Zhang JY, Zhang MY, Sugahara K, et al. Effect of steroidal saponins of Anemarrhenae rhizome on superoxide in human neutrophils[J]. Biochem Biophy RPs Comm, 1999, 259(3):636.
    [4] 韩 兵,李春梅,李 敏,等.知母皂苷的降脂及抗动脉粥样硬化作用[J]. 上海中医药杂志, 2006,40(11):68.
    [5] Takeda Y, Togashi H, Matsuo T, et al. Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhen asphodeloides Bunge[J]. J Gastroenterol, 2001, 36(2):79.
    [6] 吉 星,冯毅凡. 知母中皂苷类成分研究进展[J]. 中草药, 2010,41(4):12.
    [7] Lee B, Jung K, Kim DH. Timosaponin AⅢ, a saponin isolated from Anemarrhena asphodeloides, ameliorates learning and memory defcits in mice[J]. Pharmacol, Biochem and Behav, 2009, 93(2):121.
    [8] 沙东旭,刘兆妍,张满来,等. HPLC-ELSD测定知母中知母皂苷BⅡ的含量[J]. 药物分析杂志, 2009,29(12):2106.
    [9] 陈千良,孙小明,王文全,等. HPLC-ELSD法同时测定知母药材中2种皂苷含量[J]. 中国中药杂志, 2011,36(4):474.
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Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2012.06.010

Abstract: Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of four major steroid glycosides (Timosaponin B, Timosaponin E1, Timosaponin BⅡ and Timosaponin AⅢ). Methods HPLC analysis was performed on an Agilent Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase of water (adjust to pH 3.3 by acetic acid) (A) and acetonitrile (B). The gradient elution program was as follow:0~8 min, 12%~23% B;8~25 min, 23% B;25~35 min, 23%~45% B;35~40 min, 45%~95% B. The temperature of drift tube was 55℃ and the nebulizer nitrogen flow rate was 4.0 Bar. Results The linearity was obtained over 42.10~252.6 μg/ml (r=0.999 3) for Timosaponin B, 48.80~292.8 μg/ml (r=0.999 1) for Timosaponin E1, 192.2~1153 μg/ml (r=0.999 7) for Timosaponin BⅡ and 8.512~85.12 μg/ml (r=0.998 5) for Timosaponin AⅢ. The RSDs of precision and stability of the samples were both less than 1% in 48 hours. The average recovery was between 96.04%~102.8%. Conclusions The present method, with satisfactory efficacy, was simple which could simultaneously determine multiple steroid glycosides in Anemarrhena asphodeloides Bge. from different areas.

YIN Cha, WU Ting-ting, ZHU Dong-liang, LIU Zhi-rui, CHAI Yi-feng. Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010
Citation: YIN Cha, WU Ting-ting, ZHU Dong-liang, LIU Zhi-rui, CHAI Yi-feng. Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010
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