Decreasing effect of semen Trichosanthis oil on blood glucose on diabetic mice
-
摘要: 目的 研究瓜蒌子油对糖尿病小鼠模型的降血糖作用及其可能的作用机制。 方法 ICR小鼠尾静脉注射四氧嘧啶(100 mg/kg)造成糖尿病模型,给予不同剂量的瓜蒌子油灌胃,另设正常对照组、模型对照组和阳性药对照组,连续给药4周,每周监测小鼠体重及血糖,末次给药18 h后,摘眼球采血,分离血清,用化学比色法测血清总胆固醇(TC)、三酰甘油(TG)、一氧化氮(NO)和一氧化氮合酶(NOS)含量,用放射免疫法测血清胰岛素含量;另采用四氧嘧啶糖尿病小鼠对瓜蒌子油进行单次给药的糖耐量测定。 结果 给予瓜蒌子油不同剂量(5、10、20 ml/kg)的各组小鼠体重增长略有加快,并使血糖值呈剂量依赖性下降,能显著升高模型小鼠的血清胰岛素含量,降低血清TC、TG、NO和NOS含量;从糖耐量试验看,瓜蒌子油不同剂量组小鼠喂以淀粉后各时段血糖值呈剂量依赖性降低。 结论 瓜蒌子油具有降血糖以及改善糖耐量的作用,其降血糖的作用可能与升高血清胰岛素含量、降低血清的NO和NOS水平有关。Abstract: Objective To study the hypoglycemic activity of semen Trichosanthis oil(STO) and the mechanism on diabetic mice. Methods The alloxan-induced diabetic mice were divided into different groups and given different dose of STO by gavage for 4 weeks, the changes of body weight and fasting blood glucose were investigated every week. Blood serum was drawn to determine TC, TG, NO, NOS and insulin. In addition, the effect of STO on glucose tolerance were also investigated in alloxan-induced diabetic mice. Results STO can dose dependently reduce the levels of blood glucose and increase the body weight when it was administrated orally at 5,10,20 ml·kg-1, can increase insulin and lower TC,TG,NO,NOS in blood serum, and also can improve glucose tolerance in alloxan-induced diabetic mice. Conclusion STO may potentiate the hypoglycemic effect,and can improve glucose tolerance. The hypoglycemic activity of STO may be related with increasing insulin and decreasing NO and NOS in blood serum.
-
Key words:
- semen Trichosanthis oil /
- hypoglycemic effect /
- alloxan /
- glucose tolerance
-
[1] 钟 鑫.《药性赋》释义[J].开卷有益:求医问药,2011,4:36. [2] 张信岳,李 钦,陈爱君,等.活性糖元保降血糖作用实验研究[J].中国临床药理学与治疗学,2008,13(8):847-851. [3] Talukder FZ,Khan KA,Uddin R, et al. In vitro free radical scavenging and anti-hyperglycemic activities of Achyranthes aspera extract in alloxan-induced diabetic mice[J]. Drug Discov Ther, 2012,6(6):298-305. [4] Robertson RP.Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes[J]. J Biol Chem, 2004, 279(41):2351-2354. [5] 王 煜,李秀钧,邬云红,等. 二甲双胍对糖脂中毒的大鼠胰岛β细胞胰岛素分泌功能的保护作用[J]. 华西医学,2009,24(4):920-922. [6] 黄先菊,王文英,王贵林,等. 蛇葡萄素对2型糖尿病大鼠NO/NOS水平及抗氧化能力的影响[J]. 中国现代应用药学,2008,25(2):95-98. [7] 王玉梅,胡燕芬,肖 怀.天然药物中降血糖成分及作用机理的研究进展[J].中国民族民间医药杂志,2008,17(7):15-17. [8] 卢 威,秦晓改,王跃虎,等.二氢杨梅素对四氧嘧啶性糖尿病小鼠的降糖作用研究[J].中药药理与临床,2011,27(4):15-17. [9] 周燕文,时雪峰.柚皮苷对四氧嘧啶致糖尿病小鼠的降糖与抗氧化作用[J].中国药师,2012,15(3):293-296. [10] 周赐琴.可食用栝楼籽营养成分分析与栝楼籽油O/W微乳液的制备[D].杭州:浙江大学,2010. [11] 乐嘉静,李湛君,徐康森. 海狗油ω-3多不饱和脂肪酸对胰岛素抵抗型糖尿病大鼠的降糖作用研究[J]. 中国临床药理学与治疗学,2005,10(3):321. [12] 李国栋,范 伟,陆国浩,等.月见草油的研究概况[J].药学实践杂志,2003,21(3):171. [13] 董凤芹,李 红.一氧化氮和糖尿病血管病变[J].中华内分泌代谢杂志,1999,15(3):175. -
点击查看大图
计量
- 文章访问数: 6164
- HTML全文浏览量: 370
- PDF下载量: 986
- 被引次数: 0
下载:
