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JI Song-gang, LIU Yue, MAO Fei, LI Xiang, CHAI Yi-feng. Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 437-439,472. doi: 10.3969/j.issn.1006-0111.2012.06.011
Citation: JI Song-gang, LIU Yue, MAO Fei, LI Xiang, CHAI Yi-feng. Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 437-439,472. doi: 10.3969/j.issn.1006-0111.2012.06.011

Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS

doi: 10.3969/j.issn.1006-0111.2012.06.011
  • Received Date: 2012-09-05
  • Rev Recd Date: 2012-09-27
  • Objective To analyze chemical constituents of Scutellaria barbata D.Don by high pressure liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A SHISEIDO CAPCELL PAK C18 (100 mm×3.0 mm, 3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 15 major constituents in Scutellaria barbata D.Don were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of Scutellaria barbata D.Don.
  • [1] 王桂玲, 房建强, 赵雪梅,等. 半枝莲中总生物碱的提取和纯化条件研究[J]. 中国现代应用药学, 2011, 28(5):399.
    [2] Lee TK, Kim DI, Song YL, et al. Differential inhibition of Scutellaria barbata D. Don (Lamiaceae) on HCG-promoted proliferation of cultured uterine leiomyomal and myometrial smooth muscle cells[J]. Immunopharmacology and immunotoxicology, 2004, 26(3):329.
    [3] 陶伟. 中药半枝莲的药理及临床应用研究进展[J]. 海军医学杂志, 2008, 29(2):181.
    [4] 邹箴蕾, 吴启南. 半枝莲的化学成分及药理作用研究进展[J]. 时珍国医国药, 2005, 16(2):149.
    [5] 仲浩, 薛晓霞, 姚庆强. 半枝莲化学成分的研究[J]. 中草药, 2008, 39(1):21.
    [6] 李萍, 左甜甜, 王晓秋,等. 半枝莲的化学成分研究(Ⅱ)[J]. 中国药物化学杂志, 2008, 18(5):374.
    [7] 何枢衡, 祎张, 葛丹丹,等. 中药半枝莲黄酮类成分的分离与结构鉴定[J]. 沈阳药科大学学报, 2011, 28(3):182.
    [8] 涂琪顺, 蔡光明, 何群,等. HPLC法测定半枝莲中二萜类生物碱scutebarbatine B[J]. 中草药, 2008, 39(2):280.
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Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS

doi: 10.3969/j.issn.1006-0111.2012.06.011

Abstract: Objective To analyze chemical constituents of Scutellaria barbata D.Don by high pressure liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A SHISEIDO CAPCELL PAK C18 (100 mm×3.0 mm, 3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 15 major constituents in Scutellaria barbata D.Don were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of Scutellaria barbata D.Don.

JI Song-gang, LIU Yue, MAO Fei, LI Xiang, CHAI Yi-feng. Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 437-439,472. doi: 10.3969/j.issn.1006-0111.2012.06.011
Citation: JI Song-gang, LIU Yue, MAO Fei, LI Xiang, CHAI Yi-feng. Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 437-439,472. doi: 10.3969/j.issn.1006-0111.2012.06.011
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