Study of anti-fatigue effect of methylphenidate hydrochloride oral fast dissolving films and its mechanism
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摘要: 目的 研究盐酸哌甲酯(methylphenidate hydrochloride, MPH)口腔速溶膜(oral fast dissolving films, OFDF)抗疲劳作用及其机制。 方法 将60只小鼠随机分为6组:正常对照组(生理盐水)、游泳对照组(生理盐水)、益气养血口服液阳性组(7 mg/kg)、MPH-OFDF高、中、低剂量组(5.2、 2.6、1.3 mg/kg)。正常对照组、游泳对照组及阳性组小鼠予以灌胃,其他组小鼠每天舌下给药1次,连续15 d,末次给药30 min后进行负重游泳实验,记录小鼠游泳至力竭的时间,并检测小鼠血清乳酸脱氢酶(LDH)、肌酸激酶(CK)和三酰甘油(TG)水平。 结果 与游泳对照组比较,高、中剂量MPH-OFDF可显著延长小鼠的力竭性游泳时间(P<0.05,P<0.01),降低小鼠血清中LDH、CK水平(P<0.05,P<0.01),且中剂量MPH能降低小鼠血清的TG含量(P<0.05)。 结论 MPH-OFDF具有明显的抗疲劳作用,其机制可能与降低血清LDH、CK及TG含量有关。Abstract: Objective To study the anti-fatigue effect of methylphenidate hydrochloride oral fast dissolving films (MPH-OFDF) and its mechanism. Methods 60 mice were randomly divided into 6 groups as: normal control group (physiological saline), model group (physiological saline), Yiqiyangxue oral liquids positive group (7.00 mg/kg), MPH-OFDF high-dose group (5.20 mg/kg), MPH-OFDF middle-dose group (2.60 mg/kg) and MPH-OFDF low-dose group (1.30 mg/kg). Besides the normal control group,model group and positive group were orally administered, the other groups are administered with the drug once daily sublingually daily for consecutive 15 days. The mice were put in the load-weighted swimming test 30 min after the last oral administration, then the anti-fatigue effect was assessed based on recording exhausting swimming time and detecting the levels of serum lactale dehydrogenase (LDH), creatine kinase (CK), triglycerides (TG) in mice. Results Compared with control group, the middle-dose and the high-dose MPH could prolong the exhausting swimming time (P<0.05,P<0.01) and decrease the activity of LDH and CK significantly (P<0.05,P<0.01);in addition the middle-dose MPH could decrease the content of TG (P<0.05). Conclusion The MPH had marked anti-fatigue effect that may be associated with reduced serum LDH, CK and TG.
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[1] 戴淑萍, 卢漓江, 马爱民. 哌甲酯的临床应用现状 [J]. 中国药房, 2008, 19(8): 626-627. [2] Prommer E. Methylphenidate: established and expanding roles in symptom management [J]. Am J Hosp Palliat Care, 2012, 29:483-490. [3] Lower E, Fleishman S, Cooper A, et al. A phase Ⅲ, randomized,placebo-controlled trial of the safety and efficacy of d-MPH as new treatment of fatigue and "chemobrain" in adult cancer patients [J]. Clin Oncol, 2005:23(16S). [4] Daniel B, Philippe P, Boudewijn V H, et al. Does methylphenidate reduce the symptoms of chronic fatigue syndrome? [J]. Am J Med,2006, 119(2):e23-e30. [5] Shun G, Ping S, Hai J, et al. Effect of methylphenidate in patients with cancer-related fatigue:a systematic review and meta-analysis [J]. PLOS ONE, 2014, 9(1):e84391. [6] Aggarwal J, Singh G, Saini S, et al.C.Fast dissolving films a novel approach to oral drug delivery [J]. Int Res J Pharm, 2011, 2(12):69-74. [7] Radiloff D, Zhao Y, Boico A, et al. Anti-hypotensive treatment and endothelin blockade synergistically antagonize exercise fatigue in rats under simulated high altitude [J]. PLOS One, 2014, 9(6):e99309. [8] Thomas DP, Marshall KI. Effect of repeated exhaustive exercise on myocardial subcellular membrane structure [J]. Sports Med, 1988, (9):257-260. [9] 徐志刚. 抗疲劳药物的研究进展[J]. 海峡药学, 2012, 24(1): 21-23. [10] 张颖捷, 杜万红. 国内外抗疲劳研究进展 [J]. 实用预防医学, 2012, 19(7):1112-1116. [11] John B, Michael JM, Patricia AR, et al. Imparied calcium pum function does not slow relaxation inhuman skeletal muscle after prolonged exercise [J]. J AppIy Physiol, 1997, 83:511-521. [12] Walberg JL, Greenwood MRC, Stern JS. Lipoprotein lipase activity and lpolysis after swim taining in obese Zueker rats [J]. Am J Physiol, 1983, 245:706-712. -
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