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HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012
Citation: HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

doi: 10.3969/j.issn.1006-0111.2015.03.012
  • Received Date: 2014-12-18
  • Rev Recd Date: 2015-01-28
  • Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.
  • [1] 范 越,田 明,王秀海,等. 栀子豉汤临床和实验研究进展[J]. 中医药学报, 2010, 38(1): 118-119.
    [2] 田义龙,赵 静,任艳青, 等. 栀子豉汤对胰岛素抵抗的改善作用及机制研究[J]. 中药药理与临床, 2010,(6):5-7.
    [3] 张超云,谢东霞. 栀子豉汤治疗抑郁症有效部位的筛选研究[J]. 中华中医药刊, 2011, 29(11): 2493-2494.
    [4] 袁圣龙.栀子豉汤在心身疾病治疗中的应用[J]. 云南中医中药杂志, 2013, 34(6): 76-77.
    [5] 杨军宣,赵成城,刘 昊. 栀子环烯醚萜苷的大孔吸附树脂纯化工艺研究[J].中草药, 2012, 43(9): 1756-1759.
    [6] 彭 柳,范开静,熊清平. 大孔树脂分离纯化栀子提取液中栀子苷的工艺优选[J]. 中国实验方剂学杂志, 2012, 18(22): 20-24.
    [7] 郭文勇,刘彬果,钟 蕾, 等. 大孔树脂吸附层析法提取淡豆豉总异黄酮的研究[J]. 第二军医大学学报,2004, 25(9):1003-1034.
    [8] 王虹玉,喇孝瑾,白素芬,等. 正交试验法优选栀子豉汤的水提工艺[J]. 中国实验方剂学杂志,2012,18(22): 36-38.
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The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

doi: 10.3969/j.issn.1006-0111.2015.03.012

Abstract: Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012
Citation: HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012
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