HAN Dan, WANG Yang-ping, BI Ya-jing, LIU Fu-qiang. Extracting method optimization and physical and chemical properties of polysaccharide in Bletilla striata[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 35-37. doi: 10.3969/j.issn.1006-0111.2013.01.010
| Citation:
|
HAN Dan, WANG Yang-ping, BI Ya-jing, LIU Fu-qiang. Extracting method optimization and physical and chemical properties of polysaccharide in Bletilla striata[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 35-37. doi: 10.3969/j.issn.1006-0111.2013.01.010
|
Extracting method optimization and physical and chemical properties of polysaccharide in Bletilla striata
- 1.
School of Pharmacy, Yan Bian University, Yanji 133000, China
- 2.
The 208th Hospital of PLA, Changchun 130062, China
- Received Date: 2012-09-04
- Rev Recd Date:
2012-11-12
-
Abstract
Objective To establishing the best extraction technology of polysaccharide in Bletilla striata and researching its physical and chemical properties. Methods Water extraction and buck formulation, cellulose enzyme hydrolysis and ultrasonic extraction were used, and the spectrophotometry was used to determine the content of polysaccharide, the receiving rate of polysaccharide in the highest method as the foundation, the contents of polysaccharide as the target to process optimization. The physical and chemical characteristics of polysaccharide were studied according to the Ch.P (2010). Results The ultrasonic extraction method was the best extraction technologywith the condition that: the ratio of material and liquid was 1:20, temperature was 80℃, in 10 min.The extraction rate was 49.90%. Five physical and chemical properties were calculated. Conclusion The extraction rate of ultrasonic method for polysaccharide in Bletilla striata was high than other methods, and suitable for mass productionwhich could provide the references for modern application of polysaccharide in Bletilla striata.
-
References
|
[1]
|
孙达锋,史劲松,张卫明,等.白芨多糖胶研究进展[J].食品科学,2009,30(3):296. |
|
[2]
|
刘光斌,黄 忠,黄长干,等.白芨多糖提取工艺的研究[J].中国现代应用化学杂志,2007,24(4):289. |
|
[3]
|
舒任庚,蒋跃平,蔡永恒.植物多糖的提取分离方法探讨[J].中国药房,2011,22(11):1052. |
|
[4]
|
张楷正,杨跃寰,李光辉,等.香菇多糖提取方法研究进展[J].四川理工学院学报,2009,22(6):63. |
|
[5]
|
闫巧娟,韩鲁佳,江正强.纤维素酶解法提取黄芪多糖[J].中草药,2005,36(12):1804. |
|
[6]
|
田河林,韦立顺,悦随士.白芨胶的提取及理化特性测定[J].中国临床医药研究杂志,2004,(115):12903. |
|
[7]
|
朱小平,吴东儒.白芨多糖分离、纯化、组成及其性质[J].安徽大学学报,1991,(4):86. |
|
[8]
|
魏绍云,齐慧玲,王继伦,等.苯酚-硫酸法测定白芨多糖[J].天津化工,2000,(3):35. |
|
[9]
|
陈景耀,吴国荣,王建安,等.正交试验优选白芨多糖硫酸酯化工艺的研究[J].中草药,2005,36(1):43. |
|
[10]
|
中国药典一部[S]. 2010年版. |
-
-
Proportional views
-
DownLoad: