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用HPLC法测定丹参素棕榈醇酯脂质体药物含量

王晶 张川 栗意 任金妹 唐扣明

王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
引用本文: 王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
Citation: WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012

用HPLC法测定丹参素棕榈醇酯脂质体药物含量

doi: 10.3969/j.issn.1006-0111.2019.05.012
基金项目: 上海市卫生和计划生育委员会科研项目资助(20164Y0101)

Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method

  • 摘要: 目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。 方法 采用Agilent ZORBAX Eclipse Plus C18柱(4.6 mm×200 mm,5 μm);以乙腈-水(50∶50)含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相,流速为1 ml/min,检测波长为280 nm,柱温为室温。 结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求,在0.05~0.45 mg/ml范围内线性关系良好(r=0.999 5,n=5),回收率在96%~102%之间,日内及日间RSD均小于2%(n=5)。3批样品的含量分别为97.81%、101.20%、98.53%。 结论 该方法准确可靠、简单快速,可用于丹参素棕榈醇酯脂质体含量的测定。
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    [3] WU L,QIAO H,LI Y,et al.Protective roles of puerarin and Danshensu on acute ischemic myocardial injury in rats[J].Phytomedicine,2007,14(10):652-658.
    [4] YIN Y,GUAN Y,DUAN J L,et al.Cardioprotective effect of Danshensu against myocardial ischemia/reperfusion injury and inhibits apoptosis of H9c2 cardiomyocytes via Akt and ERK1/2 phosphorylation[J].Eur J Pharmacol,2013,699(1-3):219-226.
    [5] LAM F F,YEUNG J H,CHAN K M,et al.Relaxant effects of danshen aqueous extract and its constituent Danshensu on rat coronary artery are mediated by inhibition of calcium channels[J].Vascul Pharmacol,2007,46(4):271-277.
    [6] TANG Y Q,WANG M H,LE X Y,et al.Antioxidant and cardioprotective effects of Danshensu (3-(3,4-dihydroxyphenyl)-2-hydroxy-propanoic acid from Salvia miltiorrhiza) on isoproterenol-induced myocardial hypertrophy in rats[J].Phytomedicine,2011,18(12):1024-1030.
    [7] LE X Y.Effects of danshensu on the incidence of ischemia-reperfusion induced arrhythmia in hypertrophy rat heart[J].Chin J Nat Med,2009,6(6):461-465.
    [8] WANG F,LIU Y Y,LIU L Y,et al.The attenuation effect of 3,4-dihydroxy-phenyl lactic acid and salvianolic acid B on venular thrombosis induced in rat mesentery by photochemical reaction[J].Clin Hemorheol Microcirc,2009,42(1):7- 18.
    [9] TANG Y Q,WANG M H,CHEN C L,et al.Cardiovascular protection with Danshensu in spontaneously hypertensive rats[J].Biol Pharm Bull,2011,34(10):1596-1601.
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用HPLC法测定丹参素棕榈醇酯脂质体药物含量

doi: 10.3969/j.issn.1006-0111.2019.05.012
    基金项目:  上海市卫生和计划生育委员会科研项目资助(20164Y0101)

摘要: 目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。 方法 采用Agilent ZORBAX Eclipse Plus C18柱(4.6 mm×200 mm,5 μm);以乙腈-水(50∶50)含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相,流速为1 ml/min,检测波长为280 nm,柱温为室温。 结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求,在0.05~0.45 mg/ml范围内线性关系良好(r=0.999 5,n=5),回收率在96%~102%之间,日内及日间RSD均小于2%(n=5)。3批样品的含量分别为97.81%、101.20%、98.53%。 结论 该方法准确可靠、简单快速,可用于丹参素棕榈醇酯脂质体含量的测定。

English Abstract

王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
引用本文: 王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
Citation: WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
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