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基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

范加腾 陆峰 许激扬

范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
引用本文: 范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
Citation: FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

doi: 10.3969/j.issn.1006-0111.2019.02.011

Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS

  • 摘要: 目的 建立动态表面增强拉曼光谱法(D-SERS)快速表征两性霉素B (AMB)对白色念珠菌的抑制作用。 方法 采用D-SERS法,选用敏感的白色念珠菌分别与不同浓度的AMB相互作用,在不同的作用时间下,通过考察拉曼位移在656 cm-1和729 cm-1处的峰强比和药物浓度之间的关系,分析AMB对白色念珠菌拉曼光谱的影响。 结果 在一定药物浓度和作用时间下,峰强比与AMB浓度呈负相关,可用于描述AMB对白色念珠菌的抑制作用,且药物浓度低于其最小抑菌浓度。 结论 该方法简便、快捷,实现了AMB对白色念珠菌的快速表征,同时为表征更多药物-病原体相互作用提供了更简单、快速的检测方法。
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基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

doi: 10.3969/j.issn.1006-0111.2019.02.011

摘要: 目的 建立动态表面增强拉曼光谱法(D-SERS)快速表征两性霉素B (AMB)对白色念珠菌的抑制作用。 方法 采用D-SERS法,选用敏感的白色念珠菌分别与不同浓度的AMB相互作用,在不同的作用时间下,通过考察拉曼位移在656 cm-1和729 cm-1处的峰强比和药物浓度之间的关系,分析AMB对白色念珠菌拉曼光谱的影响。 结果 在一定药物浓度和作用时间下,峰强比与AMB浓度呈负相关,可用于描述AMB对白色念珠菌的抑制作用,且药物浓度低于其最小抑菌浓度。 结论 该方法简便、快捷,实现了AMB对白色念珠菌的快速表征,同时为表征更多药物-病原体相互作用提供了更简单、快速的检测方法。

English Abstract

范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
引用本文: 范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
Citation: FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011
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