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基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究

王学雷 晁玉凡 高松燕 董昕 温晓飞

王学雷, 晁玉凡, 高松燕, 董昕, 温晓飞. 基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究[J]. 药学实践与服务, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
引用本文: 王学雷, 晁玉凡, 高松燕, 董昕, 温晓飞. 基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究[J]. 药学实践与服务, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
WANG Xuelei, CHAO Yufan, GAO Songyan, DONG Xin, WEN Xiaofei. Urinary metabolomics study on mice renal injury caused by calcium oxalate crystal based on UPLC-Q-TOF/MS platform[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
Citation: WANG Xuelei, CHAO Yufan, GAO Songyan, DONG Xin, WEN Xiaofei. Urinary metabolomics study on mice renal injury caused by calcium oxalate crystal based on UPLC-Q-TOF/MS platform[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006

基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究

doi: 10.3969/j.issn.1006-0111.2019.02.006
基金项目: 上海市浦东新区科技发展基金(PKJ2013-Y21);上海市科学技术委员会科研计划项目(15401900800)

Urinary metabolomics study on mice renal injury caused by calcium oxalate crystal based on UPLC-Q-TOF/MS platform

  • 摘要: 目的 通过研究草酸钙结石小鼠尿液中代谢物的变化,探究草酸钙结晶导致肾损伤的内在机制。 方法 以乙醛酸盐诱导的小鼠草酸钙结晶模型为对象,采用基于超高效液相-四极杆飞行时间串联质谱(UPLC-Q-TOF/MS)的代谢组学方法测定尿液中内源性代谢物的变化,并采用SIMCA-P进行多元统计分析,Metabo Analyst软件进行代谢物通路分析。 结果 与正常组相比,模型组小鼠的肾组织出现明显的钙盐沉积且血清中的肌酐和尿素氮含量异常升高,肾脏出现损伤;从尿液中筛选出尿酸、牛磺酸、苯丙氨酸等21个差异代谢物。 结论 通过代谢物通路分析,差异代谢物主要涉及氨基酸代谢、能量代谢、牛磺酸代谢、嘌呤代谢和VB6代谢,为进行结石疾病机制研究以及早期标志物的筛选提供了重要参考。
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基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究

doi: 10.3969/j.issn.1006-0111.2019.02.006
    基金项目:  上海市浦东新区科技发展基金(PKJ2013-Y21);上海市科学技术委员会科研计划项目(15401900800)

摘要: 目的 通过研究草酸钙结石小鼠尿液中代谢物的变化,探究草酸钙结晶导致肾损伤的内在机制。 方法 以乙醛酸盐诱导的小鼠草酸钙结晶模型为对象,采用基于超高效液相-四极杆飞行时间串联质谱(UPLC-Q-TOF/MS)的代谢组学方法测定尿液中内源性代谢物的变化,并采用SIMCA-P进行多元统计分析,Metabo Analyst软件进行代谢物通路分析。 结果 与正常组相比,模型组小鼠的肾组织出现明显的钙盐沉积且血清中的肌酐和尿素氮含量异常升高,肾脏出现损伤;从尿液中筛选出尿酸、牛磺酸、苯丙氨酸等21个差异代谢物。 结论 通过代谢物通路分析,差异代谢物主要涉及氨基酸代谢、能量代谢、牛磺酸代谢、嘌呤代谢和VB6代谢,为进行结石疾病机制研究以及早期标志物的筛选提供了重要参考。

English Abstract

王学雷, 晁玉凡, 高松燕, 董昕, 温晓飞. 基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究[J]. 药学实践与服务, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
引用本文: 王学雷, 晁玉凡, 高松燕, 董昕, 温晓飞. 基于UPLC-Q-TOF/MS平台的结晶肾损伤小鼠的尿液代谢组学研究[J]. 药学实践与服务, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
WANG Xuelei, CHAO Yufan, GAO Songyan, DONG Xin, WEN Xiaofei. Urinary metabolomics study on mice renal injury caused by calcium oxalate crystal based on UPLC-Q-TOF/MS platform[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
Citation: WANG Xuelei, CHAO Yufan, GAO Songyan, DONG Xin, WEN Xiaofei. Urinary metabolomics study on mice renal injury caused by calcium oxalate crystal based on UPLC-Q-TOF/MS platform[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 126-134. doi: 10.3969/j.issn.1006-0111.2019.02.006
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