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海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究

唐杰 林厚文 孙凡

唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
引用本文: 唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
Citation: TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004

海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.004
基金项目: 国家自然科学青年基金项目(81502936)

Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells

  • 摘要: 目的 研究海绵Spongia pertusa Esper来源的smenospongine(Sme)诱导乳腺癌细胞MCF7凋亡的作用机制。 方法 使用CCK-8法检测Sme对MCF7细胞活力的影响;DAPI染色检测凋亡细胞的细胞核形态;使用流式细胞术检测细胞凋亡率和线粒体膜电位;Western blotting检测Sme对Bax、Bcl2、细胞色素C(cytochorome C)、p-p38和p38蛋白水平表达的影响。 结果 CCK-8法检测结果表明,Sme抑制MCF7增殖,IC50值为(16.46 ±0.88)μmol/L;DAPI染色结果和Annexin V-FITC/PI染色结果显示Sme诱导细胞凋亡。随着加药浓度的增加,细胞凋亡率从4.18%逐渐升至21.49%;流式细胞仪检测结果表明Sme引发MCF7细胞内线粒体膜电位下降;Western blotting结果显示Sme激活了内源性凋亡途径和p38丝裂源活化蛋白激酶(MAPK)信号通路。 结论 Sme可能通过激活p38 MAPK通路,诱导细胞内源性凋亡发挥抗乳腺癌作用。
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    [2] MOLINSKI TF, DALISAY DS, LIEVENS SL, et al. Drug development from marine natural products[J]. Nat Rev Drug Discov,2009, 8(1):69-85.
    [3] LI J, GU BB, SUN F, et al. Sesquiterpene quinones/hydroquinones from the Marine Sponge Spongia pertusa Esper[J],J Nat Prod,2017, 80(5):1436-1445.
    [4] KONG D, AOKI S, SOWA Y, et al. Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leukemia cells[J]. Mar Drugs, 2008, 6(3):480-488.
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    [6] AOKI S, KONG D, MATSUI K, et al. Sesquiterpene aminoquinones, from a marine sponge, induce erythroid differentiation in human chronic myelogenous leukemia, K562 cells[J]. Chem Pharm Bull (Tokyo),2004, 52(8):935-937.
    [7] KONG D, YAMORI T, KOBAYASHI M, et al. Antiproliferative and antiangiogenic activities of smenospongine, a marine sponge sesquiterpene aminoquinone[J]. Mar Drugs,2011, 9(2):154-161.
    [8] SMITH RA, COKKINIDES V, BROOKS D, et al. Cancer screening in the United States, 2010:a review of current American Cancer Society guidelines and issues in cancer screening[J]. CA Cancer J Clin,2010, 60(2):99-119.
    [9] QUEIROZ EA, PUUKILA S, EICHLER R, et al. Metformin induces apoptosis and cell cycle arrest mediated by oxidative stress, AMPK and FOXO3a in MCF-7 breast cancer cells[J]. PLoS ONE,2014, 9(5):e98207.
    [10] KONDRACKI ML, GUYOT M. Smenospongine:a cytotoxic and antimicrobial aminoquinone isolated from ja:math sp[J]. Tetrahedron Letters,1987, 28(47):5815-5818.
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  • [1] 修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星.  全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 1-6. doi: 10.12206/j.issn.2097-2024.202312054
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海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.004
    基金项目:  国家自然科学青年基金项目(81502936)

摘要: 目的 研究海绵Spongia pertusa Esper来源的smenospongine(Sme)诱导乳腺癌细胞MCF7凋亡的作用机制。 方法 使用CCK-8法检测Sme对MCF7细胞活力的影响;DAPI染色检测凋亡细胞的细胞核形态;使用流式细胞术检测细胞凋亡率和线粒体膜电位;Western blotting检测Sme对Bax、Bcl2、细胞色素C(cytochorome C)、p-p38和p38蛋白水平表达的影响。 结果 CCK-8法检测结果表明,Sme抑制MCF7增殖,IC50值为(16.46 ±0.88)μmol/L;DAPI染色结果和Annexin V-FITC/PI染色结果显示Sme诱导细胞凋亡。随着加药浓度的增加,细胞凋亡率从4.18%逐渐升至21.49%;流式细胞仪检测结果表明Sme引发MCF7细胞内线粒体膜电位下降;Western blotting结果显示Sme激活了内源性凋亡途径和p38丝裂源活化蛋白激酶(MAPK)信号通路。 结论 Sme可能通过激活p38 MAPK通路,诱导细胞内源性凋亡发挥抗乳腺癌作用。

English Abstract

唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
引用本文: 唐杰, 林厚文, 孙凡. 海绵来源的smenospongine诱导乳腺癌MCF7细胞凋亡机制研究[J]. 药学实践与服务, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
Citation: TANG Jie, LIN Houwen, SUN Fan. Effect of marine sponge-derived smenospongine on apoptosis in breast cancer MCF7 cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 399-402,421. doi: 10.3969/j.issn.1006-0111.2018.05.004
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