Quality standards for Kangkeling mixture
-
摘要: 目的 建立康咳灵合剂的质量标准。 方法 采用薄层色谱法(TLC)对矮地茶、百部进行定性鉴别;采用高效液相色谱法(HPLC)测定岩白菜素的含量。色谱柱为Lichrospher C18柱(4.6 mm×250 mm,5 μm);流动相:甲醇-水(20:80);检测波长:275 nm;柱温:30℃;流速:1 ml/min。 结果 TLC法能准确鉴别矮地茶和百部,斑点清晰;岩白菜素在0.064 8~0.648 μg(r=0.9998)范围内线性关系良好,平均回收率100.24%(RSD为1.9%,n=6)。 结论 本实验建立的方法简便、专属性高、重复性好,结果准确可靠,可用于康咳灵合剂的质量控制。Abstract: Objective To establish quality standards for Kangkeling mixture. Methods Ardisiae Japonicae Herba, Stemonae Radix were identified by TLC. The content of bergenin was determined by HPLC. Lichrospher C18 column(5 μm,4.6 mm×250 mm)was used with methanol-water (20:80) as mobile phase. The flow rate was 1.0 ml/min. Column temperature was 30℃ and the detection wavelength at 275 nm. Results The linear range of bergenin was in the range of 0.064 8-0.648 μg (r=0.999 8). The average recovery was 100.24% (RSD=1.9%, n=6). Conclusion The method is simple, specific and sensitive. It can be used to control the quality of Kangkeling mixture.
-
Key words:
- Kangkeling mixture /
- TLC /
- HPLC /
- quality standard
-
[1] 刘伟林, 杨东爱, 余胜民, 等. 矮地茶药理作用研究[J]. 时珍国医国药, 2009, 20(12):3002-3003. [2] 陈向东, 张光大, 刘法锦, 等. HPLC法同时测定矮地茶配方颗粒中岩白菜素和槲皮苷[J]. 广东药学院学报, 2012, 28(5):522-525. [3] 董成梅,杨丽川,邹澄,等. 岩白菜素的研究进展[J]. 昆明医学院学报,2012,(1):150-154. [4] 王春艳,张立新,宋成君.垂盆草颗粒质量标准研究[J].黑龙江医药,2009,22(2):128-130. [5] 胡君萍, 张囡, 毛一卿,等. 《中国药典》 3种百部的止咳作用比较[J], 中国中药杂志, 2009, 34(23):3096-3104. [6] 国家药典委员会.中华人民共和国药典2015年版一部[S].北京:中国医药科技出版社,2015:361.
计量
- 文章访问数: 3417
- HTML全文浏览量: 358
- PDF下载量: 689
- 被引次数: 0