Quantitation of nine ginsenosides in Panax ginseng hairy roots and other Panax ginseng plant components by HPLC
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摘要: 目的 采用HPLC法测定不同人参样品中9种人参皂苷（Rc、Rb1、Rb2、Re、Rd、Rg1、Rg2、Rg3和Rh2）的含量。 方法 色谱条件：Zorbax SB C18柱（4.6 mm×250 mm，5μm），保护柱Extend-C18柱（4.6 mm×12.5 mm，5μm）；以乙腈-水为流动相，梯度洗脱；流速：1.0 ml/min；检测波长：203 nm；柱温：35℃。 结果 9种人参皂苷Rg1、Rb1、Re、Rc、Rg2、Rh2、Rg3、Rb2和Rd在120 min内基线分离。方法学表明其线性关系良好，精密度、稳定性和重复性RSD均小于2.0%，加样回收率在98.3%～102%之间。测得人参叶和人参须根中的总皂苷含量最高，分别为48.9、23.6 mg/g；人参毛状根中的总皂苷与人参主根和人参果的总皂苷含量差别不大，为7.47 mg/g。 结论 该法准确性高，操作简便、快速，重复性好，精密度高，可用于不同人参样品中9种人参皂苷的含量测定。Abstract: Objective To establish an HPLC method for the quantitation of nine ginsenosides (Rc,Rb1,Rb2,Re,Rd,Rg1,Rg2,Rg3 and Rh2) in Panax ginseng samples. Methods An HPLC method was developed to determine the quantities of the nine ginsenosides. The determination was performed on a Zorbax SB C18 column (4.6 mm×250 mm,5 μm) with an Extend-C18 guard column (4.6 mm×12.5 mm,5 μm) at 35℃. The mobile phase was a multi-step acetonitrile-water gradient run at a flow rate of 1.0 ml/min. The detection wavelength was 203 nm. Results The nine ginsenosides were baseline separated within 120 min. The method had good linearity, precision, stability and reproducibility with RSDs all less than 2.0%. The sample recoveries were between 98.3% to 102%. The quantity of total saponins in leaves and fibrous roots of Panax ginseng, which were measured as 48.9 mg/g and 23.6 mg/g, respectively, were higher than those in the other plant components. The amounts of total saponins in Panax ginseng hairy roots were similar to those in taproots and fruits of Panax ginseng, which was 7.47 mg/g. Conclusion The established HPLC method is accurate, simple, rapid, precise and reproducible and could be used for the quantitation of these nine ginsenosides in Panax ginseng samples.
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Key words:
- Panax ginseng decoction pieces /
- hairy root /
- Panax ginseng /
- ginsenoside /
- HPLC
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