Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra
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摘要: 目的 建立红参药材高效液相色谱(HPLC)指纹图谱,为其质量评价提供依据。 方法 Sharpsil-T C18色谱柱(4.6 mm×250 mm,5 μm);以乙腈-水为流动相梯度洗脱;柱温为30℃;流速为1.0 ml/min;检测波长为203 nm。 结果 以人参皂苷Rb1为参照峰,建立10批红参的对照指纹图谱,识别出24个共有峰,其相似度均大于0.940。通过质谱推断、对照品比对确证了其中11个共有峰。 结论 该方法操作简便、准确可靠、重复性较好,为红参药材的质量控制提供了有效手段。Abstract: Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control. Methods Sharpsil-T C18 column(4.6 mm×250 mm, 5 μm)was used with acetonitrile-water in gradient elution mode. The flow rate was 1.0 ml/min, the column temperature was maintained at 30℃, and the detective wavelength was 203 nm. Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chromatographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak. The similarity was more than 0.940. Eleven chemical compounds were identified by comparing the reference substance. Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision, stability, and reproducibility.
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Key words:
- Ginseng Radix et Rhizoma Rubra /
- HPLC /
- fingerprint
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[1] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:143. [2] 罗国安,王明义,曹进,等.建立我国现代中药质量标准体系的研究[J].世界科学技术-中药现代化,2002,4(4):5-11. [3] 刘唯芬,熊英,幕善学,等.红参HPLC指纹图谱研究[J].辽宁中医杂志,2009,36(7):1178-1179. [4] 蔡萍,肖娟,张水寒,等.红参超微饮片的指纹图谱研究[J].中华中医药杂志,2011,26(7):1513-1515. [5] 郑重,宋凤瑞,刘淑莹,等.人参、红参皂苷类成分指纹图谱研究[J].质谱学报,2012,33(6):327-333.
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