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氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用

付慧 陶玉龙 倪敏 李冬洁 沈甫明

付慧, 陶玉龙, 倪敏, 李冬洁, 沈甫明. 氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用[J]. 药学实践与服务, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
引用本文: 付慧, 陶玉龙, 倪敏, 李冬洁, 沈甫明. 氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用[J]. 药学实践与服务, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
FU Hui, TAO Yulong, NI Min, LI Dongjie, SHEN Fuming. Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
Citation: FU Hui, TAO Yulong, NI Min, LI Dongjie, SHEN Fuming. Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005

氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用

doi: 10.3969/j.issn.1006-0111.2016.03.005
基金项目: 国家重大新药创制(2011ZXJ09201-012)

Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine

  • 摘要: 目的 研究氯喹(chloroquine, CQ),及CQ是否增敏化疗药物地塞米松(dexamethasone, DEX)或辐射对多发性骨髓瘤细胞株U266细胞的杀伤作用,并探讨其可能机制。 方法 用细胞活性检测试剂盒(cell counting kit-8, cck8)检测CQ单药,以及CQ联用DEX对U266细胞增殖的影响,运用中效原理软件评估两药相互作用;cck8及流式细胞术分别检测CQ处理条件下辐射对U266细胞增殖和凋亡作用的改变;Western blot检测CQ合用DEX,以及CQ联合辐射对U266细胞内B细胞淋巴瘤-2(B-cellymphoma-2, Bcl-2)蛋白表达变化。 结果 CQ及DEX对U266细胞的增殖抑制作用呈浓度依赖性,且CQ(3.9 μmol/L)能够增强DEX(125 μmol/L)对U266细胞的杀伤作用,并增加DEX降低细胞内抗凋亡蛋白Bcl-2的表达水平;不同辐射剂量(5~25 Gy)对U266细胞的生长抑制作用并无差异;CQ(1.0 μmol/L)可以增加辐射对U266细胞的敏感性,诱导细胞凋亡。 结论 CQ能够增敏DEX或辐射对U266细胞的杀伤作用,CQ增敏DEX的作用机制可能与抑制Bcl-2蛋白表达相关。
  • [1] Feng W, Fu Y, Zhang Y, et al. Establishment of stable multiple myeloma cell line with overexpressed PDCD5 and its proapoptosis mechanism[J]. Int J Clin Exp Pathol, 2015,8:10635-10643.
    [2] Dong H, Carlton ME, Lerner A, et al. Effect of cAMP signaling on expression of glucocorticoid receptor, Bim and Bad in glucocorticoid-sensitive and resistant leukemic and multiple myeloma cells[J]. Front Pharmacol, 2015,6:230.
    [3] Solomon VR, Lee H. Chloroquine and its analogs:a new promise of an old drug for effective and safe cancer therapies[J]. Eur J Pharmacol, 2009,625:220-233.
    [4] Fukuda T, Oda K, Wada-Hiraike O, et al. The anti-malarial chloroquine suppresses proliferation and overcomes cisplatin resistance of endometrial cancer cells via autophagy inhibition[J]. Gynecol Oncol, 2015,137:538-545.
    [5] Hu T, Li P, Luo Z, et al. Chloroquine inhibits hepatocellular carcinoma cell growth in vitro and in vivo[J]. Oncol Rep, 2015,35:43-49.
    [6] Tang MC, Wu MY, Hwang MH, et al. Chloroquine enhances gefitinib cytotoxicity in gefitinib-resistant nonsmall cell lung cancer cells[J]. PLoS One, 2015,10:e0119135.
    [7] Han MW, Lee JC, Choi JY, et al. Autophagy inhibition can overcome radioresistance in breast cancer cells through suppression of TAK1 activation[J]. Anticancer Res, 2014,34:1449-1455.
    [8] Ghafouri-Fard S, Seifi-Alan M, Shamsi R, et al. Immunotherapy in multiple myeloma using cancer-testis antigens[J]. Iran J Cancer Prev, 2015,8:e3755.
    [9] Lambert KE, Huang H, Mythreye K, et al. The type Ⅲ transforming growth factor-beta receptor inhibits proliferation, migration, and adhesion in human myeloma cells[J]. Mol Biol Cell, 2011,22:1463-1472.
    [10] 刘竞,李昕,桂嵘,等. 程序化细胞死亡分子-5蛋白对地塞米松诱导的多发性骨髓瘤细胞凋亡的影响[J]. 中国老年学杂志, 2010,30:1972-1974.
    [11] Lee SW, Kim HK, Lee NH, et al. The synergistic effect of combination temozolomide and chloroquine treatment is dependent on autophagy formation and p53 status in glioma cells[J]. Cancer Lett, 2015,360:195-204.
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    [13] 袁晓丽,李涛,黄建鸣,等. 氯喹对食管癌细胞系放射增敏的实验研究[J]. 中国放射医学与防护杂志, 2014,34:823-826.
    [14] MS Ola MN, Ahsan H. Role of Bcl-2 family proteins and caspases in the regulation of apoptosis[J]. Mol Cell Biochem, 2011,351:41-58.
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  • 收稿日期:  2016-03-09
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氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用

doi: 10.3969/j.issn.1006-0111.2016.03.005
    基金项目:  国家重大新药创制(2011ZXJ09201-012)

摘要: 目的 研究氯喹(chloroquine, CQ),及CQ是否增敏化疗药物地塞米松(dexamethasone, DEX)或辐射对多发性骨髓瘤细胞株U266细胞的杀伤作用,并探讨其可能机制。 方法 用细胞活性检测试剂盒(cell counting kit-8, cck8)检测CQ单药,以及CQ联用DEX对U266细胞增殖的影响,运用中效原理软件评估两药相互作用;cck8及流式细胞术分别检测CQ处理条件下辐射对U266细胞增殖和凋亡作用的改变;Western blot检测CQ合用DEX,以及CQ联合辐射对U266细胞内B细胞淋巴瘤-2(B-cellymphoma-2, Bcl-2)蛋白表达变化。 结果 CQ及DEX对U266细胞的增殖抑制作用呈浓度依赖性,且CQ(3.9 μmol/L)能够增强DEX(125 μmol/L)对U266细胞的杀伤作用,并增加DEX降低细胞内抗凋亡蛋白Bcl-2的表达水平;不同辐射剂量(5~25 Gy)对U266细胞的生长抑制作用并无差异;CQ(1.0 μmol/L)可以增加辐射对U266细胞的敏感性,诱导细胞凋亡。 结论 CQ能够增敏DEX或辐射对U266细胞的杀伤作用,CQ增敏DEX的作用机制可能与抑制Bcl-2蛋白表达相关。

English Abstract

付慧, 陶玉龙, 倪敏, 李冬洁, 沈甫明. 氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用[J]. 药学实践与服务, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
引用本文: 付慧, 陶玉龙, 倪敏, 李冬洁, 沈甫明. 氯喹增敏地塞米松或辐射对多发性骨髓瘤细胞的杀伤作用[J]. 药学实践与服务, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
FU Hui, TAO Yulong, NI Min, LI Dongjie, SHEN Fuming. Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
Citation: FU Hui, TAO Yulong, NI Min, LI Dongjie, SHEN Fuming. Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 210-214. doi: 10.3969/j.issn.1006-0111.2016.03.005
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