盐霉素钠纳米脂质体的制备及表征

巩志荣; 何文婷; 孙治国; 郭海霞; 钟延强; 鲁莹

doi:10.3969/j.issn.1006-0111.2015.01.009

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

盐霉素钠纳米脂质体的制备及表征

巩志荣, 何文婷, 孙治国, 郭海霞, 钟延强, 鲁莹

文章导航 > 药学实践与服务 > 2015 > 33(1): 36-39,43

巩志荣, 何文婷, 孙治国, 郭海霞, 钟延强, 鲁莹. 盐霉素钠纳米脂质体的制备及表征[J]. 药学实践与服务, 2015, 33(1): 36-39,43. doi: 10.3969/j.issn.1006-0111.2015.01.009

引用本文:

GONG Zhirong, HE Wenting, SUN Zhiguo, GUO Haixia, ZHONG Yanqiang, LU Ying. Preparation and characterization of salinomycin sodium loaded nano liposomes[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 36-39,43. doi: 10.3969/j.issn.1006-0111.2015.01.009

Citation:

GONG Zhirong, HE Wenting, SUN Zhiguo, GUO Haixia, ZHONG Yanqiang, LU Ying. Preparation and characterization of salinomycin sodium loaded nano liposomes[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 36-39,43. doi: 10.3969/j.issn.1006-0111.2015.01.009

盐霉素钠纳米脂质体的制备及表征

doi: 10.3969/j.issn.1006-0111.2015.01.009

1.
第二军医大学药学院, 上海 200433
2.
解放军266医院, 河北承德 067000

Preparation and characterization of salinomycin sodium loaded nano liposomes

1.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
No.266 Hospital of PLA, Chengde 067000, China

摘要: 目的制备并表征盐霉素钠纳米脂质体(SLN)。方法采用薄膜分散法制备盐霉素钠纳米脂质体,通过调节脂质体中胆固醇比例,以盐霉素钠包封率为评价指标,筛选盐霉素钠纳米脂质体的优化处方。结果透射电镜显示盐霉素钠纳米脂质体形态圆整,分散性良好,激光粒度仪显示盐霉素钠纳米脂质体平均粒径为99.0 nm,Zeta电位为-33.5 mV,包封率为85.7%,载药量为6.7%。通过脂质体包裹,盐霉素钠在水中的最高浓度可提高15倍,并证明其具有一定缓释效果。结论笔者得到了粒径大小在100 nm左右,形态均一,包封率和载药量较高的盐霉素钠纳米脂质体,为进一步测定其杀伤肿瘤活性奠定了坚实的制剂学基础。
- 肿瘤干细胞 /
- 盐霉素钠 /
- 脂质体 /
- 包封率 /
- 处方优化
Abstract: Objective To prepare and characterize salinomycin sodium-loaded nano liposomes(SLN). Methods The nano liposomes were prepared by a thin-film dispersion method.The formula of SLN was optimized by regulating the cholesterol ratio of the nano liposomes,using the encapsulation efficacy (EE) of SLN as the primary outcome measure. Results Transmission electron microscope (TEM) showed that SLN was round and had a good dispersion.Dynamic laser scatter (DLS) showed that SLN was of a desired size of 99 nm,and zeta potential of -33.5 mV.EE of SLN was 85.7% and drug loading of 6.7%.According to the formulation of nano liposomes,the concentration of salinomycin sodium in water was greatly improved by 15 folds.Additionally,the nano liposomes were observed to exhibit sustained release characteristics. Conclusion Salinomycin sodium-loaded nanoliposomes of a desired size of about 100 nm were obtained,which were well dispersion,and high EE and drug loading.Solid pharmaceutics foundation for the activity examination of SLN was provided in this research.
- cancer stem cells /
- salinomycin sodium /
- liposomes /
- entrapment efficiency /
- optimization of preparation

[1]	Reya T,Morrison SJ,Clarke MF,et al.Stem cells,cancer,and cancer stem cells[J].Nature,2001,414(6859):105-111. [2] Dean M,Fojo T,Bates S.Tumour stem cells and drug resistance[J].Nat Rev Cancer,2005,5(4):275-284. [3] Gupta PB,Onder TT,Jiang G,et al.Identification of selective inhibitors of cancer stem cells by high-throughput screening[J].Cell,2009,138(4):645-659. [4] Naujokat C,Steinhart R.Salinomycin as a drug for targeting human cancer stem cells[J].Biomed Res Int,2012,2012:1-17. [5] Huczynski A.Salinomycin:a new cancer drug candidate[J].Chem Biol Drug Des,2012,79(3):235-238. [6] Liu Y,Miyoshi H,Nakamura M.Nanomedicine for drug delivery and imaging:a promising avenue for cancer therapy and diagnosis using targeted functional nanoparticles[J].Int J Cancer,2007,120(12):2527-2537. [7] Chang H,Yeh M.Clinical development of liposome-based drugs:formulation,characterization,and therapeutic efficacy[J].Int J Nanomed,2012,7:49-60. [8] Barenholz YC.Doxil -the first FDA-approved nano-drug:lessons learned[J].J Control Release,2012,160(2):117-134. [9] Farokhzad OC,Langer R.Nanomedicine:developing smarter therapeutic and diagnostic modalities[J].Adv Drug Deliver Rev,2006,58(14):1456-1459.
[2]	Mayer LD,Hope MJ,Cullis PR.Vesicles of variable sizes produced by a rapid extrusion procedure[J].BBA-Biomembranes 1986,858(1):161-168.
[3]	Mayer LD,Harasym TO,Tardi PG,et al.Ratiometric dosing of anticancer drug combinations:controlling drug ratios after systemic administration regulates therapeutic activity in tumor-bearing mice[J].Mol Cancer Ther,2006,5(7):1854-1863.
[4]	Damen J,Regts J,Scherphof G.Transfer and exchange of phospholipid between small unilamellar liposomes and rat plasma high density lipoproteins Dependence on cholesterol content and phospholipid composition[J].Biochim Biophys Acta,1981,665(3):538-545.
[5]	朱会琴, 逄涣欢,胡昌勤,等.浊度法测定两性霉素B及其脂质体的效价[J].中国抗生素杂志,2006,30(12):752-755.
[6]	Wong M,Chiu GN.Simultaneous liposomal delivery of quercetin and vincristine for enhanced estrogen-receptor-negative breast cancer treatment[J].Anticancer Drugs,2010,21(4):401-410.
[7]	Lasic DD,Woodle MC,Martin FJ,et al.Phase-behavior of stealth-lipid-lecithin mixtures[J].Period Biol,1991,93(2):287-290.
[8]	Dusi G,Gamba V.Liquid chromatography with ultraviolet detection of lasalocid,monensin,salinomycin and narasin in poultry feeds using pre-column derivatization[J].J Chromatogr A,1999,835(1-2):243-246.

[1]	刘丽艳, 余小翠, 孙传铎. 纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
[2]	宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅. 帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
[3]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[4]	修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星. 全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
[5]	姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量. 巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
[6]	杨媛媛, 安晓强, 许佳捷, 江键, 梁媛媛. 正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用 . 药学实践与服务, 2024, 42(6): 244-247. doi: 10.12206/j.issn.2097-2024.202310027
[7]	陈春娟, 郑志新, 李骊. 平喘方联合孟鲁司特钠治疗儿童支气管哮喘患者的临床疗效观察 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202405035
[8]	崔亚玲, 吴琼, 马良煜, 胡北, 姚东, 许子华. 肝素钠肌醇烟酸酯乳膏中肌醇烟酸酯皮肤药动学研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404006
[9]	史生辉, 石飞, 雷琼, 王亚峰, 吴雪花. 青藏高原肺结核合并念珠菌感染患者的病原菌分布特点及耐药率分析 . 药学实践与服务, 2024, 42(6): 260-262, 272. doi: 10.12206/j.issn.2097-2024.202304014
[10]	凯丽比努尔·奥布力艾散, 李倩, 谢志, 贾文彦, 尹东锋. 星点设计-效应面法优化仑伐替尼混合胶束的制备工艺 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202403019
[11]	迟文雅, 袁艳, 李伟林, 吴茼妤, 俞媛. 负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架用于创伤性脑损伤治疗 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202406034
[12]	陈炳辰, 王思真, 郭贝贝, 杨峰. 紫杉醇棕榈酸酯的合成及其脂质体的制备与处方研究 . 药学实践与服务, 2024, 42(9): 379-384, 410. doi: 10.12206/j.issn.2097-2024.202404062

点击查看大图

计量

文章访问数: 3541
HTML全文浏览量: 253
PDF下载量: 256
被引次数: 0

盐霉素钠纳米脂质体的制备及表征

doi: 10.3969/j.issn.1006-0111.2015.01.009

1. 第二军医大学药学院, 上海 200433

2. 解放军266医院, 河北承德 067000

收稿日期: 2014-09-27
修回日期: 2014-11-16

关键词:

肿瘤干细胞 /

盐霉素钠 /

脂质体 /

包封率 /

处方优化

摘要: 目的制备并表征盐霉素钠纳米脂质体(SLN)。方法采用薄膜分散法制备盐霉素钠纳米脂质体,通过调节脂质体中胆固醇比例,以盐霉素钠包封率为评价指标,筛选盐霉素钠纳米脂质体的优化处方。结果透射电镜显示盐霉素钠纳米脂质体形态圆整,分散性良好,激光粒度仪显示盐霉素钠纳米脂质体平均粒径为99.0 nm,Zeta电位为-33.5 mV,包封率为85.7%,载药量为6.7%。通过脂质体包裹,盐霉素钠在水中的最高浓度可提高15倍,并证明其具有一定缓释效果。结论笔者得到了粒径大小在100 nm左右,形态均一,包封率和载药量较高的盐霉素钠纳米脂质体,为进一步测定其杀伤肿瘤活性奠定了坚实的制剂学基础。