摘要:
目的 优化牡丹皮提取条件,建立同时测定牡丹皮中没食子酸、芍药苷和丹皮酚3种有效成分含量的反相高效液相色谱分析方法。 方法 采用Agilent Plus C18柱,以乙腈-0.6%甲酸水溶液为流动相,梯度洗脱,流速1.0 ml/min,检测波长254 nm,进样量10 μl。 结果 回归方程分别为:没食子酸Y=16.598 0X+4.950 2,r=0.999 9;芍药苷Y=1.613 1X+0.995 1,r=0.999 9;丹皮酚Y=5.981 7X+54.103 0,r=0.999 7。线性范围分别为4.0~100.1;20.6~515.0;33.3~832.0 μg/ml。平均回收率分别为100.7%、99.2%、97.8%;RSD(n=9)分别为1.6%、3.0%、1.1%。 结论 考察了牡丹皮的提取条件,提取效果良好,分析方法准确可靠,重复性好,回收率高,适用于牡丹皮的含量测定。
Abstract:
Objective To optimize the extraction methods and develop a HPLC-DAD assay for the determination of three effective ingredients in Moutan Coretex. Methods The analysis was performed on an Agilent ZORBAX Eclipse Plus (4.6 mm×150 mm, 5 μm) column with acetonitrile and water (0.6% formic acid) as mobile phase at a rate of 1.0 ml/min, the detection wavelength was 254 nm, the injection volume was 10 μl. Results The regress equation of gallic acid, paeoniflorin and paeonol were Y=16.598 0X+4.950 2, r=0.999 9; Y=1.613 1X+0.995 1, r=0.999 9; Y=5.981 7X+54.103 0, r=0.999 7, respectively. The average recoveries were 100.7%, 99.2%, 97.8% and RSD(n=9) were 1.6%, 3.0%, 1.1%. Conclusion The extraction effect was good and the determination assay was reliable and accurate.
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