摘要:
目的: 研究知母皂苷B-Ⅱ抗抑郁的作用,并初步探讨其作用机制。 方法: 采用小鼠悬尾实验、小鼠强迫游泳实验、开野实验;阿朴吗啡致小鼠刻板行为实验;抑制单胺(去甲肾上腺素NE和多巴胺DA)重摄取实验;育亨宾毒性增强实验;以及5-羟色胺酸(5-HTP)致甩头作用等动物模型来考察知母皂苷B-Ⅱ抗抑郁作用及其可能的机制。分别以小鼠不动时间、自主活动数、刻板行为评分、死亡率作为评价指标。 结果: 在小鼠悬尾实验中,知母皂苷B-Ⅱ(100、150 mg/kg)能够明显缩短小鼠的不动时间;在小鼠强迫游泳实验中,知母皂苷B-Ⅱ(50、100、150 mg/kg)均能够明显缩短小鼠的不动时间;对开野实验中小鼠的自主活动次数无明显影响。在抑制NE和DA重摄取实验中,知母皂苷B-Ⅱ高剂量(150 mg/kg)显著增加多巴胺(DA)致小鼠死亡作用,但对去甲肾上腺素(NE)重摄取抑制作用不明显。阿朴吗啡致小鼠刻板运动实验显示,知母皂苷B-Ⅱ(100、150 mg/kg)对注射阿朴吗啡后的小鼠刻板运动有显著增强作用,差异具有显著意义(P<0.05);具有增加5-羟色胺酸(5-HTP)致甩头作用;知母皂苷B-Ⅱ不具有增加育亨宾毒性的作用。 结论: 知母皂苷B-Ⅱ有抗抑郁活性,其作用机制可能与增强脑内5-HT、DA神经系统作用有关。
Abstract:
Objective To study the effect and mechanisms of timosaponin B-Ⅱ on depression. Methods The tail suspension test(TST),forced swimming test(FST),open field activity test(OFA),APO-induced synergistic action test,inhibiting reuptake of monoamine neurotransmitters and monoamine oxidase inhibitor(MAOI) test,yohimbine-induced lethality test and 5-HTP-induced head-twitch test were carried out to examine the antidepressant effect and mechanisms of timosaponin B-Ⅱ in rats.Immobility duration in FST and TST,spontaneous activity frequency in OFA,the stereotyped behaviors scores in APO-induced synergistic action test,and mortality in the other tests were observed. Results Timosaponin B-Ⅱ(100、150mg/kg) could effectively shorten the immobility time in TST and FST,but all dosages showed no significant effect on the spontaneous activity frequency in OFA.Timosaponin B-Ⅱ(150 mg/kg) significantly increased the rate of death of injecting dopamine,but had no obvious effect on the inhibiting reuptake of NE.Timosaponin B-Ⅱ(100、150 mg/kg) enhanced the apomorphine-induced stereotypy remarkably,which with statistically significant differences(P<0.05),and increased the head-twitch response evoked by 5-HTP,but had no effect on the yohimbine toxicity. Conclusion Timosaponin B-Ⅱhas significant antidepressant effect which may be related to the potentiation of brain 5-HT nerve system and DA nerve system.
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