毛细管电泳法检测中药制剂舒骨宁中非法添加的双氯芬酸钾
Determination of illegal ingredient diclofenacpotassium in TCM by capillary electrophoresis
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摘要: 目的:采用毛细管电泳的方法检测中药制剂舒骨宁中非法添加的西药双氯芬酸钾。方法:毛细管电泳法,贝克曼未涂层石英毛细管柱75μm×50 cm,以25 mM的磷酸氢二钠（磷酸调节pH6.50±0.02）为缓冲液,0.5 psi、5 s压力进样,检测波长275 nm,柱温为25℃,分离电压25 kV。结果:双氯芬酸钾的含量在0.010～0.200 g/L的范围内线性关系良好,r=0.998 4,平均回收率为100.6%,RSD为3.01%。结论:该方法简便、快速、可靠,溶剂用量少,可有效检测出中药制剂舒骨宁中非法添加的西药双氯芬酸钾。Abstract: Objective :To establish a capillary electrophoresis(CE) method for determining the illegally added ingredient diclofenac potassium in TCM,Shuguning tablet. Methods : CE was used for the separation by using the uncoaded capillary(75 μm×50 cm) and buffer of 25 mmol/L Na2HPO4(pH 6.50±0.02).The applied voltage was 25 kV at 25℃.The sample was injected for 5s at 0.5psi and the detection wavelength was at 275 nm. Results :Linear range was from 0.010 g/L to 0.200 g/L(r=0.998 4),the average recovery was 100.6% with the RSD of 4.39%. Conclusion : This method is simple,rapid,reliable and accurate for quantifying the illegally added diclofenac potassium in Shuguning tablets.
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Key words:
- capillary electrophoresis /
- TCM /
- illegal ingredient /
- diclofenac potassium
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