摘要:
目的:建立咔唑法同时测定玻璃酸钠(SH)和硫酸软骨素(CS)含量的方法。方法:通过醋酸钠调节样品的酸碱度和离子强度,氯化十六烷基吡啶(CPC)可选择性地沉淀CS,而SH不沉淀,从而将SH和CS进行分离。结果:样品预处理条件为:精密量取SH和CS的混合溶液10 mL,加入醋酸钠1.6 g,12.5 mmol/LCPC10 mL,充分搅拌后,静置1 h,20μm滤膜过滤;滤液用于测定SH的含量;滤饼经1.4 mol/L氯化钠溶液超声解离后,用于测定CS的含量;SH和CS的平均回收率分别为101.2%和99.4%,RSD分别为1.05%和0.96%。结论:本法可同时测定SH和CS的含量,相互之间无干扰。
Abstract:
Objective :To establish a carbazole method for the simultaneous determination of sodium hyaluronate(SH) and chondroitin sulfate(CS). Methods : By regulating acidity or alkalinity and ionic strength with sodium acetate,CS was selectively precipitated with cetylpyridinium chloride(CPC),whereas SH was not.So SH and CS were separated. Results :The sample pretreatment conditions were as follows:Measuring 10 mL mixture of SH and CS accurately,adding 1.6 g sodium acetate and 10 mL CPC of 12.5 mmol/L,standing for 1 h after stirring thoroughly,then,the solution was filtered with 20 μm filter membrane and the filtrate was used to determine the content of SH;the precipitates on the filter membrane were resolved with 1.4 mol/L NaCl solution by ultrasonic treatment for the determination of CS.The average recoveries of SH and CS were 101.2% and 99.4% with RSD of 1.05% and 0.96%,respectively. Conclusion :This method can be used for the simultaneous determination of SH and CS without interference with each other.
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