摘要:
目的: 建立HPLC同时测定银黄口服液中绿原酸和黄芩苷含量的方法。 方法: 色谱柱用十八烷基硅烷键合硅胶为固定相（Agilent ZORBAX Eclipse XDB-C18,4.6×150 mm,5μm）。流动相:A为甲醇-水-磷酸（50:950:5）,B为甲醇;梯度洗脱程序:0-30,A:95%35%,B:5%65%;流速:1 mL/min,检测波长327 nm。 结果: 绿原酸和黄芩苷的保留时间分别约为10.5 min和23.8 min,与各自相邻峰的分离度均大于1.5。以峰面积（X）对进样浓度（Y,μg/mL）线性回归,绿原酸回归方程:Y=0.03200X-0.02160,r=0.9999,线性范围5.040-45.36μg/mL,黄芩苷回归方程:Y=0.04970X-0.4880,r=0.9999,线性范围:44.88-403.9μg/mL。绿原酸、黄芩苷回收率分别为95.7%和99.0%,RSD分别为0.43%,0.33%。 结论: 本方法回收率测定以及与药典法含量测定结果比较,显示本法测定结果准确,且操作简便,精密度好。
Abstract:
Objective: To develop a HPLC quantitative method for the determination of chlorgenic acid and baicalin in Yinhuang Oral liquid. Methods: An Agilent C18 column(ZORBAX Eclipse XDB-C18,4.6×150 mm,5 μm)was used and a mixture of methanol-water-phosphorc acid(50:950:5)was used as mobile phase A,methanol as mobile phase B,gradient elution:0～30,A:95%～35%,B:5%～65%.The flow rate was 1.0 mL/min and monitored at 327 nm. Results: The retention time of chlorgenic acid and baicalin were 10.5 min and 23.8 min,resp...
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