高效液相色谱法测定乙肝宁胶囊中芍药苷的含量
Determination of paeoniflorin in Yiganning capsule by HPLC
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摘要: 目的 建立HPLC法测定乙肝宁胶囊中芍药苷含量的高效液相色谱法。方法 采用高效液相色谱法,ZORBAX RX-SIL分析柱(150mm×4.6mm,5μm);流动相:正已烷-二氯甲烷-甲醇-浓氨水(270:270:20:1.7);检测波长:254nm(带宽2nm)参比波长:550nm(带宽100nm);流速:1.0mL/min。结果 芍药苷的线形关系良好(r=0.9995),加样回收率为97.8%,RSD<为1.9%(n=5)。结论 该方法准确、灵敏度高,重现性好。Abstract: Objective To develop a method for the determination of paeoniflorin in Yiganning capsule by HPLC has been developed. Methods Chromatographic analysis was carried out on a ZORBAX RX-SIL column (150mm×4.6mm, 5μm) with n-hexane and dichloromethane and methanol and strong ammonia water (270:270:20:1.7, volume ratio) as mobile phase. The detection wavelength was 254nm (band width 2nm) and reference wavelength 550nm (band width 100nm). 1mL of supernatant were injected. ResultsThe linear range was well (r=0.9995), the averang recovery of paeoniflorin was 97.8% (n = 5), and the relative standard deviation was 1.9%. Conclusion This method is accurate, sensible and reproducible.
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Key words:
- HPLC /
- Yiganning capsule /
- paeoniflorin
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