Preparation of cabazitaxel albumin nanoparticles and evaluation of its biocompatibility in vitro
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摘要: 目的 制备卡巴他赛白蛋白纳米粒（CBZ-BSA-Gd-NP）以降低药物毒性，并评价其体外生物相容性。 方法 采用生物矿化法制备CBZ-BSA-Gd-NP，对其处方工艺进行优化，对粒径、Zeta电位、载药量等性质进行表征，并采用体外溶血试验考察其体外血液相容性。 结果 制得的纳米粒包封率为63.04%，载药量为10.51%，平均粒径为（166.1±4.7） nm，粒径的多分散系数（PDI）为0.256，Zeta电位为（-18.14±1.16） mV，与卡巴他赛-吐温溶液相比，体外溶血作用显著降低。 结论 该方法操作简便，制备的CBZ-BSA-Gd-NP载药量高，粒径均匀，体外血液相容性好，增加了药物使用的安全性。Abstract: Objective To prepare cabazitaxel albumin nanoparticles(CBZ-BSA-Gd-NP) to reduce drug toxicity,and to evaluate its biocompatibility in vitro. Methods CBZ-BSA-Gd-NP was prepared by biomineralization method.The formulation process was optimized,and the properties such as particle size,Zeta potential and drug loading were characterized.The in vitro hemolysis characteristics were investigated. Results The encapsulation rate of the prepared nanoparticles was 63.04%,the drug loading was about 10.51%.The particle size was (166.1±4.7) nm with the PDI 0.256.The Zeta potential was (-18.14±1.16)mV.The hemolysis effect of CBZ-BSA-Gd-NP in vitro was significantly lower than that of cabazitaxel-Tween solution. Conclusion The preparation method of CBZ-BSA-Gd-NP in this study is simple,with high drug loading,uniform particle size and good blood compatibility in vitro,which will increase the safety for clinical use of the drug.
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Key words:
- cabazitaxel /
- albumin nanoparticles /
- biocompatibility /
- prostate cancer
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