留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

应中央军委要求,2022年9月起,《药学实践杂志》将更名为《药学实践与服务》,双月刊,正文96页;2023年1月起,拟出版月刊,正文64页,数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿!

色谱-质谱联用技术测定人体内类固醇激素的研究进展

钱跹 赵亮 吕磊 张海 李悦悦 张国庆

钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
引用本文: 钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
Citation: QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004

色谱-质谱联用技术测定人体内类固醇激素的研究进展

doi: 10.3969/j.issn.1006-0111.2014.03.004

Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry

  • 摘要: 类固醇激素是高效能的生物化学物质,在多种生理活动中发挥显著的调节作用,但在体内的含量极低,因此对其进行准确定量具有重要意义。在各种检测方法中,色谱-质谱联用技术因具有高效、快速、灵敏的优点而得以广泛应用。笔者综述近年来色谱-质谱联用技术在内源性类固醇激素测定中的应用,为进一步的临床研究提供参考依据。
  • [1] Holst JP,Soldin OP,Guo T,et al. Steroid hormones: relevance and measurement in the clinical laboratory[J].Clin Lab Med,2004,24(1):105-118.
    [2] Dorgan JF,Fears TR,McMahon RP,et al. Measurement of steroid sex hormones in serum: a comparison of radioimmunoassay and mass spectrometry[J].Steroids,2002,67(3-4):151-158.
    [3] Carter GD,Carter R,Jones J,et al. How accurate are assays for 25-hydroxyvitamin D-Data from the international vitamin D external quality assessment scheme[J].Clin Chem,2004,50(11):2195-2197.
    [4] Hollis BW,Kamerud JQ,Kurkowski A,et al. Quantification of circulating 1,25-dihydroxyvitamin D by radioimmunoassay with 125 I-labeled tracer[J].Clin Chem,1996,42(4):586-592.
    [5] Hsing AW,Stanczyk FZ,Belanger A,et al. Reproducibility of serum sex steroid assays in men by RIA and mass spectrometry[J].Cancer Epidemiol Biomarkers Prev,2007,16(5):1004-1008.
    [6] Poulson K,Sancho J,Haber E. A simplified radioimmunoassay for plasma aldosterone employing an antibody of unique specificity[J].Clin Immunol,1974,2(3):373-380.
    [7] Schioler V,Thode J. Six direct radioimmunoassays of estradiol evaluated[J].Clin Chem,1988,34(5):949-952.
    [8] Xu X,Roman JM,Issaq HJ,et al.Quantitative measurement of endogenous estrogens and estrogen metabolites in human serum by liquid chromatography-tandem mass spectrometry[J].Anal Chem,2007,79(20):7813-7821.
    [9] Santen RJ,Demers L,Ohorodnik S,et al. Superiority of gas chromatography/tandem mass spectrometry assay (GC/MS/MS) for estradiol for monitoring of aromatase inhibitor therapy[J].Steroids,2007,72(8):666-671.
    [10] Wolthers BG,Kraan GP. Clinical applications of gas chromatography and gas chromatographymass spectrometry of steroids[J].J Chromatogr A,1999,843(1-2):247-274.
    [11] Draisci R,Palleschi L,Ferretti E,et al.Quantitation of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2000,870(1-2):511-522.
    [12] Guo T,Chan M,Soldin SJ. Steroid profiles using liquid chromatography-tandem mass spectrometry with atmospheric pressure photoionization source[J].Arch Pathol Lab Med,2004,128(4):469-475.
    [13] Guo T,Gu J,Soldin OP,et al.Rapid measurement of estrogens and their metabolites in human serum by liquid chromatographytandem mass spectrometry without derivatization[J].Clin Biochem,2008,41(9):736-741.
    [14] Nelson RE,Grebe SK,Kane DJ,et al. Liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of estradiol and estrone in human plasma[J].Clin Chem,2004,50(2):373-384.
    [15] Guo T,Taylor RL,Singh RJ,et al. Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photospray ionization tandem mass spectrometry[J].Clin Chim Acta,2006,372(1-2):76-82.
    [16] Andrew R. Clinical measurement of steroid metabolism[J].Best Pract Res Clin Endocrinol Metab,2001,15(1):1-16.
    [17] Abraham GE. Solid-phase radioimmunoassay of 17β-estradiol[J].J Clin Endocrinol Metab, 1969,29(6):866-870.
    [18] Rahkonena PK,Huhtinenb K,Poutanenb M,et al. Fast and sensitive liquid chromatography-mass spectrometry assay for seven androgenic and progestagenic steroids in human serum[J].J Steroid Biochem Mol Biol,2011,127(3-5):396-404.
    [19] Rothman MS,Carlson NE,Xu M,et al. Reexamination of testosterone, dihydrotestosterone, estradiol and estrone levels across the menstrual cycle and in postmenopausal women measured by liquid chromatography-tandem mass spectrometry[J].Steroids,2011,76(1-2):177-182.
    [20] Kushnir MM,Rockwood AL,Roberts WL,et al. Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids[J].Clin Chem,2006,52(8):1559-1567.
    [21] Carvalho VM,Nakamura OH,Vieira JG. Simultaneous quantitation of seven endogenous C-21 adrenal steroids by liquid chromatography tandem mass spectrometry in human serum[J].J Chromatogr B Analyt Technol Biomed Life Sci,2008,872(1-2):154-161.
    [22] McWhinney BC,Briscoe SE,Ungerer JP. Pretorius CJ.Measurement of cortisol, cortisone, prednisolone, dexamethasone and 11-deoxycortisol with ultra high performance liquid chromatography-tandem mass spectrometry: application for plasma, plasma ultrafiltrate, urine and saliva in a routine laboratory[J].J Chromatogr B Analyt Technol Biomed Life Sci,2010,878(28):2863-2869.
    [23] Rauh M,Gröschl M,Rascher W,et al.Automated, fast and sensitive quantification of 17 alpha-hydroxy-progesterone, androstenedione and testosteroneby tandem mass spectrometry with on-line extraction[J].Steroids,2006,71(6):450-458.
    [24] Shackleton CH. Mass spectrometry: application to steroid and peptide research[J].Endocr Rev,1985,6(3):441-486.
    [25] Hansen M,Jacobsen NW,Nielsen FK,et al. Determination of steroid hormones in blood by GC-MS/MS[J]. Anal Bioanal Chem,2011,400(10):3409-3417.
    [26] Courant F,Aksglaede L,Antignac JP,et al. Assessment of circulating sex steroid levels in prepubertal and pubertal boys and girls by a novel ultrasensitive gas chromatography-tandem mass spectrometry method [J].J Steroid Biochem Mol Biol,2010,95(1):82-92.
    [27] Steven JS,Soldin OP. Steroid hormone analysis by tandem mass spectrometry[J].Clin Chem,2009,55(6):1061-1066.
    [28] McDonald JG,Matthew S,Richard J. Steroid profiling by gas chromatography-mass spectrometry and high performance liquid chromatography-mass spectrometry for adrenal diseases[J].Horm Cancer,2011,2(6):324-332.
    [29] Kushnir MM,Rockwood AL,William L,et al. Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories[J].Clin Biochem,2011,44 (1):77-88.
    [30] Koal T,Schmiederer D,Rauh M. Standardized LC-MS/MS based steroid hormone profile-analysis[J].J Steroid Biochem Mol Biol,2012,129(3-5):129-138.
    [31] Naessen T,Sjogren U,Bergquist J,et al. Endogenous steroids measured by high-specificity liquid chromatography-tandem mass spectrometry and prevalent cardiovascular disease in 70-year-old men and women[J].J Clin Endocrinol Metab,2010,95(4):1889-1897.
    [32] Rossi C,Calton L,Hammond G,et al. Serum steroid profiling for congenital adrenal hyperplasia using liquid chromatography-tandem mass spectrometry[J].Clin Chim Acta,2010,411(3-4):222-228.
    [33] Kulle AE,Riepe FG,Melchior D,et al. A novel ultrapressure liquid chromatography tandem mass spectrometry method for the simultaneous determination of androstenedione, testosterone, and dihydrotestosterone in pediatric blood samples: age-and sex-specific reference data[J].J Clin Endocrinol Metab,2010,95(5):2399-2409.
    [34] Rousu T,Tolonen A. Comparison of unit resolution SRM and TOF-MS at 12,000 mass resolution for quantitative bioanalysis of 11 steroids from human plasma[J].Bioanalysis,2012,4(5):555-563.
    [35] Ceglarek U,Kortz L,Leichtle A,et al. Rapid quantification of steroid patterns in human serum by on-line solid phase extraction combined with liquid chromatography-triple quadrupole linear ion trap mass spectrometry[J].Clin Chim Acta,2009,401(1-2):114-118.
    [36] Higashi T,Shimada K. Derivatization of neutral steroids to enhance their detection characteristics in liquid chromatography-mass spectrometry[J].Anal Bioanal Chem,2004,378(4):875-882.
    [37] Maidana P, Bruno OD, Mesch V. A critical analysis of cortisol measurements: an update[J]. Medicina (B Aires),2013,73(6):579-584.
  • [1] 陈炳辰, 王思真, 郭贝贝, 杨峰.  紫杉醇棕榈酸酯的合成及其脂质体的制备与处方研究 . 药学实践与服务, 2024, 42(): 1-7. doi: 10.12206/j.issn.2097-2024.202404062
    [2] 毛泽玲, 文波.  大黄的HPLC指纹图谱及禁用农药的残留研究 . 药学实践与服务, 2024, 42(7): 297-304, 314. doi: 10.12206/j.issn.2097-2024.202310057
    [3] 李清, 郭宜银, 陈颖, 瞿发林, 董文燊, 戈煜.  夜宁胶囊对小鼠镇静催眠作用及其机制的研究 . 药学实践与服务, 2024, 42(): 1-4. doi: 10.12206/j.issn.2097-2024.202211047
    [4] 张成中, 朱雪艳, 卜其涛, 王宏瑞, 黄宝康.  基于网络药理学与分子对接预测鸡骨草特征图谱研究 . 药学实践与服务, 2024, 42(7): 1-9. doi: 10.12206/j.issn.2097-2024.202303048
    [5] 张元林, 宋凯, 孙蕊, 舒飞, 舒丽芯, 杨樟卫.  基于真实世界数据的药物利用研究综述 . 药学实践与服务, 2024, 42(6): 238-243. doi: 10.12206/j.issn.2097-2024.202312010
    [6] 崔俐俊, 陈嫣婷, 费永和, 陈静.  中外药品应急审批制度对比研究 . 药学实践与服务, 2024, 42(8): 1-6. doi: 10.12206/j.issn.2097-2024.202310015
    [7] 王晓飞, 张颖, 顾佳钰, 胡馨儿, 张海, 曹岩.  表面等离子共振传感器的识别元件在医药领域中的研究应用进展 . 药学实践与服务, 2024, 42(): 1-9. doi: 10.12206/j.issn.2097-2024.202309014
    [8] 陈炳辰, 佟达丰, 万苗, 闫飞虎, 姚建忠.  UPLC-MS/MS法测定小鼠血浆中紫杉醇脂肪酸酯前药及其药代动力学研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404082
    [9] 姚小静, 计佩影, 陆峰, 施国荣, 傅翔.  表面增强拉曼光谱法快速测定尿液中曲马多的研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202401072
    [10] 赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量.  中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
    [11] 张艺昕, 关欣怡, 王博宁, 闻俊, 洪战英.  二氢吡啶类钙离子拮抗药物手性分析及其立体选择性药动学研究进展 . 药学实践与服务, 2024, 42(8): 1-6. doi: 10.12206/j.issn.2097-2024.202308062
    [12] 顾佳钰, 胡馨儿, 王晓飞, 张颖, 张海, 曹岩.  侧流免疫层析定量检测方法的研究进展 . 药学实践与服务, 2024, 42(7): 273-277, 284. doi: 10.12206/j.issn.2097-2024.202307037
    [13] 马兹芬, 许维恒, 金煜翔, 薛磊.  食管癌的靶向治疗与免疫治疗研究进展 . 药学实践与服务, 2024, 42(6): 231-237. doi: 10.12206/j.issn.2097-2024.202306008
    [14] 徐飞, 陈瑾, 鲁育含, 李志勇.  肠道菌群参与糖尿病肾病的机制研究进展 . 药学实践与服务, 2024, 42(5): 181-184, 197. doi: 10.12206/j.issn.2097-2024.202312023
    [15] 陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰.  HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202403030
    [16] 杨凤艳, 张月, 陈恩贤, 缪雪蓉, 魏凯.  瑞马唑仑临床应用研究进展 . 药学实践与服务, 2024, 42(): 1-10. doi: 10.12206/j.issn.2097-2024.202405026
    [17] 张林晨, 张小琴, 张俊平.  山楂酸药理作用的研究进展 . 药学实践与服务, 2024, 42(5): 185-189. doi: 10.12206/j.issn.2097-2024.202307052
    [18] 李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇.  HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007
    [19] 王耀振, 徐灿, 吕顺莉, 田泾, 张东炜.  钾离子竞争性酸阻滞剂的药学特征研究进展 . 药学实践与服务, 2024, 42(7): 278-284. doi: 10.12206/j.issn.2097-2024.202306040
    [20] 夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞.  核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(): 1-4. doi: 10.12206/j.issn.2097-2024.202404063
  • 加载中
计量
  • 文章访问数:  3339
  • HTML全文浏览量:  239
  • PDF下载量:  339
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-08-28
  • 修回日期:  2014-01-14

色谱-质谱联用技术测定人体内类固醇激素的研究进展

doi: 10.3969/j.issn.1006-0111.2014.03.004

摘要: 类固醇激素是高效能的生物化学物质,在多种生理活动中发挥显著的调节作用,但在体内的含量极低,因此对其进行准确定量具有重要意义。在各种检测方法中,色谱-质谱联用技术因具有高效、快速、灵敏的优点而得以广泛应用。笔者综述近年来色谱-质谱联用技术在内源性类固醇激素测定中的应用,为进一步的临床研究提供参考依据。

English Abstract

钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
引用本文: 钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
Citation: QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
参考文献 (37)

目录

    /

    返回文章
    返回