Solid-phase synthesis of melittin of anti-tumor peptide
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摘要: 目的 合成具有抗肿瘤活性的二十六肽蜂毒素。 方法 采用Fmoc固相逐步化学合成,确立了以Wang树脂为固相载体,HOBt/DCC为缩合剂的合成工艺。 结果 通过本法能够顺利合成得到二十六肽蜂毒素,收率达32%。 结论 该合成方法具有可行性强、操作简便、总收率高等特点,适用于长链多肽的合成。Abstract: Objective To synthesize the antitumor activity of twenty-six peptide melittin. Methods The melittin was synthesized by stepwise solid-phase peptide synthesis (SPPS). During the synthesis of melittin, wang-resin was chosen as solid supporter and HOBt/DCC were used as the coupling reagent. Results The melittin was synthesized with yield of 32%. Conclusion The synthetic method was feasible, simple and with high yield for long chain polypeptide synthesis.
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Key words:
- melittin /
- solid-phase peptide synthesis /
- stepwise synthesis
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