摘要:
目的 建立同时测定二维亚铁颗粒中维生素C和维生素B1含量的HPLC方法。 方法 采用Insteril ODS-3色谱柱,流动相为0.03 mol/L磷酸氢二铵溶液(加入0.01%庚烷磺酸钠,用磷酸调节pH至3.0)-甲醇,梯度洗脱,检测波长为244 nm,流速为0.6~1.0 ml/min,柱温30℃。 结果 维生素C与维生素B1分别在0.501~6.011 μg(r=1.000 0)与 0.038~0.456 μg(r=0.999 9)范围内线性关系良好,平均回收率分别为99. 51%与99.20%,RSD分别为0.55%与0.49%(n=9)。 结论 本法简便、结果准确可靠,可用于二维亚铁颗粒中维生素C和维生素B1的质量控制。
Abstract:
Objective To establish a method for the simultaneous determination of vitamin C and vitamin B1 in ferrous fumarate granules. Methods The procedure was performed on the Insteril ODS-3 C18 column at 30℃, detected at 244 nm. The mobile phase was composed by a gradient of 0.03 mol/L of ammonium phosphate solution (in which 0.01% of sodium heptanesulfonate was added and the pH was adjusted to 3.0 by phosphoric acid) and methanol, with the flux at 0.6~1.0 ml/min. Results Either vitamin C or vitamin B1 was in good linear at 0.501~6.011 μg, r=1.0000 and 0.038~0.456 μg, r=0.999 9, respectively. The mean recoveries were 99.51% and 99.20%, with the RSD 0.55% and 0.49%, n=9. Conclusion The method was proved to be simple, accurate and reliable, appropriate for the quality control for vitamin C and vitamin B1 in ferrous fumarate granules.
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