Determination of hydroxysaffor yellow A in Yihe decoction by HPLC
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摘要: 目的 建立伊赫汤中羟基红花黄色素A的含量测定方法。 方法 采用高效液相色谱法(HPLC),色谱柱为大连依力特C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.5%乙酸溶液(20:80),检测波长为403 nm,流速为1.0 ml/min。 结果 羟基红花黄色素A在0.816 0~32.64 μg/ml浓度范围内具有良好的线性关系,r=0.999 9,平均加样回收率为101.0%,RSD为1.8%(n=6)。 结论 本方法快速、准确、重复性好,可用于伊赫汤中羟基红花黄色素A的含量测定。Abstract: Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction.
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Key words:
- Yihe decoction /
- hydroxysaffor yellow A /
- HPLC
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