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作为基因输送载体的壳聚糖衍生物研究进展

李晏

李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
引用本文: 李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
Citation: LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.

作为基因输送载体的壳聚糖衍生物研究进展

Research progress of chitosan derivatives as gene delivery vector

  • 摘要: 壳聚糖作为基因载体,目前存在的主要问题是还不能达到足够高的表达效率。其中主要原因是壳聚糖在pH 7.4的生理环境下溶解度较差,壳聚糖与DNA形成的复合物在生理环境下的稳定性较差,缺乏细胞靶向性。本文综述了作为基因输送载体的壳聚糖衍生物研究进展,为进一步研究和开发壳聚糖衍生物提供依据和参考。
  • [1] Brus C, Petersen H, Aigner A, et al. Efficiency of polyethylenimines and polyethyleniminegraft-poly (ethylene glycol) block copolymers to protect oligonucleotides against enzymatic degradation[J]. Eur.J Pharm Biopharm, 2004, (57): 427.
    [2] Fischer D, Osburg B, Petersen H, et al. Effect of poly(ethyleneimine) molecular weight and pegylation on organ distribution and pharmacokinetics of polyplexes with oligodeoxynucleotides in mice[J]. Drug Metab Dispos,2004, (32): 983.
    [3] Mao S, Neu M, Germershaus O, et al. Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly(ethylene imine)-graft-poly(ethylene glycol) block copolymer/SiRNA polyplexes[J]. Bioconjug Chem, 2006 ,(17) : 1209.
    [4] Kunath K, von Harpe A, Petersen H, et al. The structure of PEG-modified poly(ethylene imines) influences biodistribution and pharmacokinetics of their complexes with NF-kappaB decoy in mice[J].Pharm Res,2002, (19) : 810.
    [5] Zhang Y, Chen J, Zhang Y, et al. A novel PEGylation of chitosan nanoparticles for gene delivery[J].Biotechnol Appl Biochem, 2007 ,(46) :197.
    [6] Zhang H, Mardyani S, Chan WC, et al.Design of biocompatible chitosan microgels for targeted pH-mediated intracellular release of cancer therapeutics[J]. Biomacromolecules,2006, (7) :1568.
    [7] Kim TH, Nah JW, Cho MH, et al. Receptor-mediated gene delivery into antigen presenting cells using mannosylated chitosan/DNA nanoparticles[J]. J Nanosci Nanotechnol,2006, (6) :2796.
    [8] Park IK, Kim TH, Park YH, et al. Galactosylated chitosan-graft-poly(ethylene glycol) as hepatocyte-targeting DNA carrier[J]. J Control Release,2001,(76): 349.
    [9] Mansouri S, Cuie Y, Winnik , et al. Characterization of folate-chitosan-DNA nanoparticles for gene therapy[J].Biomaterials,2006, (27) :2060..
    [10] Jiang H, Kwon J, Kim E, et al. Galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine as a gene carrier for hepatocyte-targeting[J]. J Control Release, 2008, (131) :150.
    [11] Schaffer DV, Lauffenburger DA, Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery[J]. J Biol Chem,1998, (273) :28004.
    [12] Benns JM, Choi JS, Mahato RI, et al. pH sensitive cationic polymer gene delivery vehicle: N-Acpoly( L-histidine)-graft-poly(L-lysine) comb shaped polymer[J]. Bioconjug Chem,2000, (11): 637.
    [13] Li W, Nicol F, Szoka Jr FC, GALA: a designed synthetic pH-responsive amphipathic peptide with applications in drug and gene delivery[J]. Adv Drug Deliv Rev, 2004, (56) :967.
    [14] Wagner E, Effects of membrane-active agents in gene delivery[J].J Control Release, 1998, (53) :155.
    [15] Jones RA, Cheung CY, Black FE, et al. Poly(2-alkylacrylic acid) polymers deliver molecules to the cytosol by pHsensitive disruption of endosomal vesicles[J]. Biochem J, 2003, (372) :65.
    [16] Kim TH, Kim SI, Akaike T, et al. Synergistic effect of poly(ethylenimine) on the transfection efficiency of galactosylated chitosan/DNA complexes[J].J Control Release, 2005, (105) :354.
    [17] Thanou M, Florea BI, Geldof M, et al. Quaternized chitosan oligomers as novel gene delivery vectors in epithelial cell lines[J]. Biomaterials, 2002, (23): 153.
    [18] Mao S, Shuai X, Unger F, et al. Synthesis, characterization and cytotoxicity of poly (ethylene glycol)-graft-trimethyl chitosan block copolymers[J]. Biomaterials, 2005,(26) : 6343.
    [19] Satoh T, Kano H, Nakatani M, et al. 6-Amino-6-deoxy chitosan. Sequential chemical modifications at the C-6 positions of N-phthaloylchitosan and evaluation as a gene carrier[J].Carbohydr Res,. 2006, (341) : 2406.
    [20] Park IK, Ihm JE, Park YH, et al. Galactosylated chitosan(GC)-graftpoly(vinyl pyrrolidone) (PVP) as hepatocytetargeting DNA carrier: preparation and physicochemical characterization of GCgraft-PVP/DNA complex (1) [J]. J Control Release,2003, (86) : 349.
    [21] Wong K, Sun G, Zhang X, et al. PEI-g-chitosan, a novel gene delivery system with transfection efficiency comparable to polyethylenimine in vitro and after liver administration in vivo[J].Bioconjug Chem,2006, (17) : 152.
    [22] Kurisawa M, Yokoyama M, Okano T, Transfection efficiency increases by incorporating hydrophobicmonomer units into polymeric gene carriers[J].J Control Release,2000, (68) : 1.
    [23] Kim YH, Gihm SH, Park CR, et al. Structural characteristics of size-controlled self aggregates of deoxycholic acidmodified chitosan and their application as a DNA delivery carrier[J].Bioconjugate Chem,2001 ,(12) : 932.
    [24] Liu WG, Zhang X, Sun SJ, et al. N-alkylated chitosan as a potential nonviral vector for gene transfection[J]. Bioconjugate Chem,2003, (14) : 782.
    [25] Hu F, Zhao M, Yuan H, et al. A novel chitosan oligosaccharidestearic acid micelles for gene delivery: properties and in vitro transfection studies[J]. Int J Pharm,2006,(315) : 158.
    [26] Mao Z, Ma L, Yan J, et al. The gene transfection efficiency of thermoresponsive N, N, N-trimethyl chitosan chloride-g-poly(N-isopropylacrylamide) copolymer[J]. Biomaterials,2007, (28) : 4488.
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  • 收稿日期:  2010-09-20
  • 修回日期:  2010-10-21

作为基因输送载体的壳聚糖衍生物研究进展

摘要: 壳聚糖作为基因载体,目前存在的主要问题是还不能达到足够高的表达效率。其中主要原因是壳聚糖在pH 7.4的生理环境下溶解度较差,壳聚糖与DNA形成的复合物在生理环境下的稳定性较差,缺乏细胞靶向性。本文综述了作为基因输送载体的壳聚糖衍生物研究进展,为进一步研究和开发壳聚糖衍生物提供依据和参考。

English Abstract

李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
引用本文: 李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
Citation: LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
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